{"id":3199,"date":"2020-06-26T16:56:26","date_gmt":"2020-06-26T07:56:26","guid":{"rendered":"http:\/\/m12n08.jp\/blog\/?p=3199"},"modified":"2024-12-30T13:32:59","modified_gmt":"2024-12-30T04:32:59","slug":"%e6%9c%88%e9%96%9350000pv%e3%83%96%e3%83%ad%e3%82%ac%e3%83%bc%e3%81%ae%e6%9c%88%e9%96%93%e3%83%96%e3%83%ad%e3%82%b0%e5%8f%8e%e5%85%a515775%e5%86%86%ef%bc%882018%e5%b9%b410%e6%9c%88%ef%bc%89-2-2-2-2-19","status":"publish","type":"post","link":"http:\/\/m12n08.jp\/blog\/share\/4588oncolys\/3199.html","title":{"rendered":"\u30aa\u30f3\u30b3\u30ea\u30b9IR\u3055\u308c\u305f\u8ad6\u6587\u90a6\u8a33\uff082020.06.26\uff09\/MYCN\u6291\u5236\u3092\u4ecb\u3057\u305f\u30c6\u30ed\u30e1\u30e9\u30fc\u30bc\u6a19\u7684\u816b\u760d\u30a6\u30a4\u30eb\u30b9\u306b\u3088\u308bMYCN\u5897\u5e45\u795e\u7d4c\u82bd\u816b\u7d30\u80de\u306e\u6392\u9664"},"content":{"rendered":"<p>\u3053\u3093\u306b\u3061\u306f\u3001\u30b7\u30a8\u30f3\uff08<strong><a href=\"https:\/\/x.com\/M12n08Jp\" target=\"_blank\" rel=\"nofollow noopener\">@M12n08Jp<\/a><\/strong>)\u3067\u3059\u3002<\/p>\n<p>IR\u3055\u308c\u305f\u8ad6\u6587\u306eDeepL\u3067\u306e\u7ffb\u8a33\u3067\u3059\u3002<\/p>\n<p>\u8ad6\u6587\u30da\u30fc\u30b8\uff08\u539f\u6587\uff1a\u82f1\u8a9e\uff09\u2192 <a href=\"https:\/\/www.sciencedirect.com\/science\/article\/pii\/S2372770520300784?via%3Dihub#bib6\" target=\"_blank\" rel=\"nofollow noopener noreferrer\">https:\/\/www.sciencedirect.com\/science\/article\/pii\/S2372770520300784?via%3Dihub#bib6<\/a><\/p>\n<div style=\"padding: 16px; border: solid 3px #9f9f9f; border-radius: 5px; background-color: #fff0f5; margin-top: 30px; margin-bottom: 30px;\"><strong>\u3010\u30c4\u30a4\u30fbY\u677f\u3067\u306e\u7c21\u5358\u306a\u307e\u3068\u3081\u30b3\u30e1\u30f3\u30c8\u3011<\/strong><\/p>\n<ul>\n<li>\u8981\u3059\u308c\u3070\u3001OBP301\u3068OBP702\u304c\u5c0f\u5150\u764c\u306b\u3082\u7d76\u5927\u306a\u52b9\u679c\u304c\u3042\u3063\u305f\u3068\u3044\u3046\u8ad6\u6587\u306a\u306e\u3067\u3059\u306d\uff01<\/li>\n<li>OBP-301\u304a\u3088\u3073OBP-702\u306f\u3001NB\u7d30\u80de\u306b\u304a\u3051\u308b\u30aa\u30fc\u30c8\u30d5\u30a1\u30b8\u30fc\u306b\u95a2\u9023\u3057\u3066\u5f37\u529b\u306a\u6297\u816b\u760d\u52b9\u679c\u3092\u793a\u3057\u307e\u3057\u305f\u3002<\/li>\n<li>OBP-301\u304a\u3088\u3073OBP-702\u306f\u3001\u76ae\u4e0bCHP-134\u816b\u760d\u306e\u5897\u6b96\u3092\u6709\u610f\u306b\u6291\u5236\u3057\u307e\u3057\u305f\u3002<\/li>\n<li>OBP-301\u3068\u6bd4\u8f03\u3057\u3066\u3001OBP-702\u306f\u691c\u67fb\u3057\u305f\u3059\u3079\u3066\u306eNB\u7d30\u80de\u682a\u306b\u5bfe\u3057\u3066\u3088\u308a\u5f37\u3044\u6297\u816b\u760d\u52b9\u679c\u3092\u793a\u3057\u305f\u3002<\/li>\n<li>OBP-301\u304a\u3088\u3073OBP-702\u306e\u983b\u7e41\u306a\u6295\u4e0e\u304c\u3001MYCN\u3067\u5897\u5e45\u3055\u308c\u305fNB\u816b\u760d\u306e\u9664\u53bb\u306b\u4f7f\u7528\u3059\u308b\u305f\u3081\u306e\u9ad8\u3044\u6cbb\u7642\u53ef\u80fd\u6027\u3092\u79d8\u3081\u3066\u3044\u308b\u3053\u3068\u3092\u793a\u5506\u3057\u3066\u3044\u307e\u3059\u3002<\/li>\n<\/ul>\n<\/div>\n<p><!--more--><\/p>\n<div style=\"padding: 16px; border: solid 3px #9f9f9f; border-radius: 5px; background-color: #fff0f5; margin-top: 30px; margin-bottom: 30px;\"><strong>\u3010\u5c0f\u5150\u304c\u3093\u3068\u306f\u3011<\/strong><\/p>\n<p>\u5c0f\u5150\u304c\u3093\u306f\u3001\u5c0f\u5150\u304c\u304b\u304b\u308b\u3055\u307e\u3056\u307e\u306a\u304c\u3093\u306e\u7dcf\u79f0\u3067\u3059\u3002\u4e00\u822c\u7684\u306b\u306f15\u6b73\u672a\u6e80\u306b\u307f\u3089\u308c\u308b\u304c\u3093\u306e\u3053\u3068\u3067\u3059\u3002<\/p>\n<p>\u4e3b\u306a\u5c0f\u5150\u304c\u3093\u306f\u3001\u767d\u8840\u75c5\u3001\u8133\u816b\u760d\u3001\u30ea\u30f3\u30d1\u816b\u3001\u795e\u7d4c\u82bd\u816b\u3057\u3093\u3051\u3044\u304c\u3057\u3085\u3001\u80da\u306f\u3044\u7d30\u80de\u816b\u760d\u30fb\u6027\u817a\u816b\u760d\u306a\u3069\u3067\u3059\u3002\u8840\u6db2\u306e\u304c\u3093\u3067\u3042\u308b\u767d\u8840\u75c5\u3084\u30ea\u30f3\u30d1\u816b\u3092\u9664\u304d\u3001\u5927\u4eba\u3067\u306f\u307e\u308c\u306a\u3082\u306e\u3070\u304b\u308a\u3067\u3059\u3002<\/p>\n<p>\u795e\u7d4c\u82bd\u816b\u3001\u814e\u82bd\u816b\uff08\u30a6\u30a3\u30eb\u30e0\u30b9\u816b\u760d\uff09\u3001\u809d\u82bd\u816b\u306a\u3069\u300c\u82bd\u816b\u300d\u3068\u547c\u3070\u308c\u308b\u304c\u3093\u306e\u539f\u56e0\u306f\u3001\u80ce\u5150\u306e\u4f53\u306e\u795e\u7d4c\u3084\u814e\u81d3\u3001\u809d\u81d3\u3001\u7db2\u819c\u3082\u3046\u307e\u304f\u306a\u3069\u306b\u306a\u308b\u306f\u305a\u3060\u3063\u305f\u7d30\u80de\u304c\u3001\u80ce\u5150\u306e\u4f53\u304c\u3067\u304d\u3042\u304c\u3063\u305f\u5f8c\u3082\u6b8b\u3063\u3066\u3044\u3066\u3001\u7570\u5e38\u306a\u7d30\u80de\u306b\u5909\u5316\u3057\u3001\u3075\u3048\u3066\u3044\u3063\u305f\u7d50\u679c\u3068\u8003\u3048\u3089\u308c\u3066\u3044\u307e\u3059\u3002\u5927\u4eba\u306e\u304c\u3093\u3068\u306f\u7570\u306a\u308a\u3001\u751f\u6d3b\u7fd2\u6163\u306b\u304c\u3093\u306e\u767a\u751f\u539f\u56e0\u304c\u3042\u308b\u3068\u8003\u3048\u3089\u308c\u308b\u3082\u306e\u306f\u5c11\u306a\u304f\u3001\u7db2\u819c\u82bd\u816b\u3084\u30a6\u30a3\u30eb\u30e0\u30b9\u816b\u760d\u306e\u3088\u3046\u306b\u3001\u4e00\u90e8\u907a\u4f1d\u3059\u308b\u3082\u306e\u3082\u3042\u308a\u307e\u3059\u3002<\/p>\n<\/div>\n<p>\u4ee5\u4e0b\u3001\u90a6\u8a33\uff08DeepL\u3067\u306e\u7ffb\u8a33\uff09<\/p>\n<h2>\u90a6\u8a33<\/h2>\n<p>\u795e\u7d4c\u82bd\u816b\uff08NB\uff09\u306f\u672b\u68a2\u4ea4\u611f\u795e\u7d4c\u7cfb\u306e\u539f\u767a\u6027\u60aa\u6027\u816b\u760d\u3067\u3042\u308b\u3002\u9ad8\u30ea\u30b9\u30af\u306e\u795e\u7d4c\u82bd\u816b\u306f\u3001MYCN\u5897\u5e45\u3068\u30d2\u30c8\u30c6\u30ed\u30e1\u30e9\u30fc\u30bc\u9006\u8ee2\u5199\u9175\u7d20\uff08hTERT\uff09\u518d\u914d\u5217\u3092\u7279\u5fb4\u3068\u3057\u3001hTERT\u6d3b\u6027\u5316\u306b\u5bc4\u4e0e\u3057\u3001\u4e88\u5f8c\u4e0d\u826f\u3068\u306a\u308b\u3002\u6211\u3005\u306f\u3001hTERT\u6d3b\u6027\u5316\u816b\u760d\u3092\u6a19\u7684\u3068\u3059\u308b\u305f\u3081\u306b\u3001hTERT\u30d7\u30ed\u30e2\u30fc\u30bf\u30fc\u304c\u816b\u760d\u7279\u7570\u7684\u306a\u30a6\u30a4\u30eb\u30b9\u8907\u88fd\u306e\u305f\u3081\u306e\u30a6\u30a4\u30eb\u30b9E1\u907a\u4f1d\u5b50\u306e\u767a\u73fe\u3092\u99c6\u52d5\u3059\u308b2\u3064\u306e\u30aa\u30f3\u30b3\u30ed\u30a4\u30c9\u6027\u30a2\u30c7\u30ce\u30a6\u30a4\u30eb\u30b9\u3001OBP-301\u3068\u816b\u760d\u6291\u5236\u5264p53\u3092\u642d\u8f09\u3057\u305fOBP-702\u3092\u958b\u767a\u3057\u305f\u3002\u672c\u7814\u7a76\u3067\u306f\u3001\u9ad8\u3044hTERT\u767a\u73fe\u3092\u793a\u30594\u3064\u306e\u30d2\u30c8MYCN\u5897\u5e45NB\u7d30\u80de\u682a\uff08IMR-32, CHP-134, NB-1, LA-N-5\uff09\u3092\u7528\u3044\u3066\u3001hTERT\u304c\u99c6\u52d5\u3059\u308b\u30aa\u30f3\u30b3\u30ed\u30a4\u30c9\u6027\u30a2\u30c7\u30ce\u30a6\u30a4\u30eb\u30b9OBP-301\u304a\u3088\u3073OBP-702\u306e\u6cbb\u7642\u52b9\u679c\u3092\u5b9f\u8a3c\u3057\u305f\u3002\u305d\u306e\u7d50\u679c\u3001OBP-301\u3068OBP-702\u306fNB\u7d30\u80de\u306e\u30aa\u30fc\u30c8\u30d5\u30a1\u30b8\u30fc\u306b\u95a2\u9023\u3057\u3066\u5f37\u3044\u6297\u816b\u760d\u52b9\u679c\u3092\u793a\u3057\u305f\u3002\u307e\u305f\u3001\u30a6\u30a4\u30eb\u30b9\u3092\u4ecb\u3057\u305fE2F1\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u6d3b\u6027\u5316\u306b\u3088\u308aMYCN\u306e\u767a\u73fe\u304c\u6291\u5236\u3055\u308c\u305f\u3002OBP-301\u304a\u3088\u3073OBP-702\u306f\u3001\u76ae\u4e0bCHP-134\u816b\u760d\u306e\u5897\u6b96\u3092\u6709\u610f\u306b\u6291\u5236\u3057\u305f\u3002\u3053\u306e\u3088\u3046\u306b\u3001\u3053\u308c\u3089\u306ehTERT\u99c6\u52d5\u578b\u30aa\u30f3\u30b3\u30ed\u30a4\u30c9\u30a2\u30c7\u30ce\u30a6\u30a4\u30eb\u30b9\u306f\u3001E2F1\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u6291\u5236\u3092\u4ecb\u3057\u305fMYCN\u767a\u73fe\u6291\u5236\u3092\u4ecb\u3057\u3066\u3001MYCN\u5897\u5e45\u3057\u305fNB\u7d30\u80de\u3092\u6392\u9664\u3059\u308b\u305f\u3081\u306e\u6709\u671b\u306a\u6297\u816b\u760d\u5264\u3067\u3042\u308b\u3068\u8003\u3048\u3089\u308c\u305f\u3002<br \/>\n<img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-3201\" src=\"http:\/\/m12n08.jp\/blog\/wp-content\/uploads\/2020\/06\/2020-06-26_163955.jpg\" alt=\"\" width=\"619\" height=\"541\" srcset=\"http:\/\/m12n08.jp\/blog\/wp-content\/uploads\/2020\/06\/2020-06-26_163955.jpg 619w, http:\/\/m12n08.jp\/blog\/wp-content\/uploads\/2020\/06\/2020-06-26_163955-137x120.jpg 137w\" sizes=\"(max-width: 619px) 100vw, 619px\" \/><\/p>\n<h2>\u5e8f\u7ae0<\/h2>\n<p>\u795e\u7d4c\u82bd\u816b\uff08NB\uff09\u306f\u3001\u672b\u68a2\u4ea4\u611f\u795e\u7d4c\u7cfb\u306e\u60aa\u6027\u816b\u760d\u3067\u3042\u308b1 \u3002NB\u306f\u6700\u3082\u4e00\u822c\u7684\u306a\u982d\u84cb\u5916\u56fa\u5f62\u816b\u760d\u3067\u3042\u308a\u3001\u5c0f\u5150\u304c\u3093\u306e\u5168\u6b7b\u4ea1\u8005\u6570\u306e15\uff05\u3092\u5360\u3081\u3066\u3044\u308b2 \u3002\u4f4e\u30ea\u30b9\u30af\u306eNB\u816b\u760d\u306f\u81ea\u7136\u306b\u6d88\u5931\u307e\u305f\u306f\u826f\u6027\u816b\u760d\u306b\u5206\u5316\u3059\u308b\u306e\u306b\u5bfe\u3057\u3001\u9ad8\u30ea\u30b9\u30af\u306eNB\u816b\u760d\u306f\u6025\u901f\u306b\u9032\u884c\u3057\u3001\u6d78\u6f64\u6027\u304a\u3088\u3073\u8ee2\u79fb\u6027\u306e\u8868\u73fe\u578b\u3092\u793a\u30593 \u3002MYCN\u306f\u9ad8\u30ea\u30b9\u30af\u306e\u4e73\u817a\u816b\u760d\u306b\u304a\u3051\u308b\u9032\u884c\u6027\u75be\u60a3\u304a\u3088\u3073\u4e88\u5f8c\u4e0d\u826f\u3068\u5f37\u304f\u95a2\u9023\u3057\u3066\u3044\u308b\u305f\u3081\u30014 MYCN\u306f\u9ad8\u30ea\u30b9\u30af\u306e\u4e73\u817a\u816b\u760d\u306e\u60aa\u6027\u5ea6\u3092\u7dad\u6301\u3059\u308b\u4e0a\u3067\u4e2d\u5fc3\u7684\u306a\u5f79\u5272\u3092\u679c\u305f\u3057\u3066\u3044\u308b\u3068\u8003\u3048\u3089\u308c\u3066\u304a\u308a\u3001MYCN\u306f\u3053\u306e\u75be\u60a3\u306e\u6cbb\u7642\u306e\u305f\u3081\u306e\u9b45\u529b\u7684\u306a\u6cbb\u7642\u6a19\u7684\u3067\u3042\u308b\u3053\u3068\u304c\u793a\u5506\u3055\u308c\u3066\u3044\u307e\u30595\u3002\u4e00\u65b9\u3001\u6700\u8fd1\u306e\u5305\u62ec\u7684\u306a\u30b2\u30ce\u30e0\u89e3\u6790\u306e\u7d50\u679c\u3001\u30d2\u30c8\u30c6\u30ed\u30e1\u30e9\u30fc\u30bc\u9006\u8ee2\u5199\u9175\u7d20\uff08hTERT\uff09\u518d\u914d\u5217\u304c\u30cf\u30a4\u30ea\u30b9\u30afNB\u816b\u760d\u306e\u65b0\u305f\u306a\u4e88\u5f8c\u56e0\u5b50\u3067\u3042\u308b\u3053\u3068\u304c\u793a\u5506\u3055\u308c\u305f7,8 \u3002\u8208\u5473\u6df1\u3044\u3053\u3068\u306b\u3001MYCN\u5897\u5e45\u3068hTERT\u518d\u914d\u5217\u3092\u793a\u3059\u30cf\u30a4\u30ea\u30b9\u30afNB\u816b\u760d\u306f\u3001hTERT\u767a\u73fe\u306e\u8ee2\u5199\u6d3b\u6027\u5316\u3068\u985e\u4f3c\u3057\u305f\u7279\u5fb4\u3092\u6709\u3057\u3066\u3044\u308b\u3002\u3057\u305f\u304c\u3063\u3066\u3001\u9ad8hTERT\u767a\u73fe\u306f\u3001MYCN\u5897\u5e45\u304a\u3088\u3073\/\u307e\u305f\u306fhTERT\u518d\u914d\u5217\u3092\u4f34\u3046\u9ad8\u30ea\u30b9\u30afNB\u306e\u6cbb\u7642\u306e\u305f\u3081\u306e\u65b0\u3057\u3044\u6cbb\u7642\u6a19\u7684\u3068\u306a\u308b\u53ef\u80fd\u6027\u304c\u3042\u308b\u3002<\/p>\n<p>9 hTERT \u3092\u6d3b\u6027\u5316\u3057\u305f\u60aa\u6027\u816b\u760d\u7d30\u80de\u3092\u6a19\u7684\u3068\u3059\u308b\u305f\u3081\u306b\u3001\u6211\u3005\u306f 2 \u7a2e\u985e\u306e hTERT \u99c6\u52d5\u578b\u30aa\u30f3\u30b3\u30ed\u30a4\u30c9\u30a2\u30c7\u30ce\u30a6\u30a4\u30eb\u30b9\u3001OBP-301\uff08\u30c6\u30ed\u30e1\u30e9\u30a4\u30b7\u30f3\uff0910 \u3068\u816b\u760d\u6291\u5236\u5264 p53 \u3092\u767a\u73fe\u3059\u308b OBP-702,11 \u3092\u958b\u767a\u3057\u3001hTERT \u30d7\u30ed\u30e2\u30fc\u30bf\u30fc\u304c\u816b\u760d\u7279\u7570\u7684\u306a\u30a6\u30a4\u30eb\u30b9\u8907\u88fd\u306e\u305f\u3081\u306e E1 \u907a\u4f1d\u5b50\u3092\u99c6\u52d5\u3057\u305f\u3002OBP-301\u304a\u3088\u3073OBP-702\u306f\u3001hTERT\u3092\u767a\u73fe\u3059\u308b\u69d8\u3005\u306a\u30d2\u30c8\u764c\u7d30\u80de\u306b\u304a\u3044\u3066\u3001\u30aa\u30fc\u30c8\u30d5\u30a1\u30b8\u30fc\u3084\u30a2\u30dd\u30c8\u30fc\u30b7\u30b9\u306b\u4f34\u3046\u6eb6\u8840\u6b7b\u3092\u8a98\u5c0e\u3059\u308b\u6cbb\u7642\u52b9\u679c\u304c\u3042\u308b\u3053\u3068\u304c\u793a\u3055\u308c\u3066\u3044\u307e\u305911, 12, 13, 14\u3002\u3055\u3089\u306b\u3001\u524d\u81e8\u5e8a\u8a66\u9a13\u3067\u306f\u3001OBP-301\u304c\u653e\u5c04\u7dda\u6cbb\u7642\u306b\u5bfe\u3057\u3066\u816b\u760d\u7d30\u80de\u3092\u611f\u4f5c\u3059\u308b\u3053\u3068\u304c\u793a\u3055\u308c\u3066\u304a\u308a16\u3001\u73fe\u5728\u3001\u98df\u9053\u764c\u60a3\u8005\u3092\u5bfe\u8c61\u306b\u3001\u653e\u5c04\u7dda\u6cbb\u7642\u3068\u306e\u4f75\u7528\u306b\u3088\u308bOBP-301\u306e\u7b2cI\/II\u76f8\u8a66\u9a13\u304c\u9032\u884c\u4e2d\u3067\u3042\u308b\u3002\u9ad8\u30ea\u30b9\u30af\u306eNB\u816b\u760d\u306f\u9ad8\u3044hTERT\u767a\u73fe\u3092\u793a\u3059\u305f\u3081\u3001hTERT\u3092\u6a19\u7684\u3068\u3057\u305f\u30aa\u30f3\u30b3\u30ed\u30a4\u30c9\u7642\u6cd5\u306f\u3001\u3053\u306e\u75be\u60a3\u306e\u6cbb\u7642\u306b\u7406\u60f3\u7684\u306a\u6297\u816b\u760d\u6226\u7565\u3067\u3042\u308b\u53ef\u80fd\u6027\u304c\u3042\u308b\u3002<\/p>\n<p>\u672c\u7814\u7a76\u3067\u306f\u3001MYCN\u5897\u5e45NB\u7d30\u80de\u306b\u5bfe\u3059\u308bhTERT\u99c6\u52d5\u578b\u30aa\u30f3\u30b3\u30ed\u30a4\u30c9\u30a2\u30c7\u30ce\u30a6\u30a4\u30eb\u30b9OBP-301\u304a\u3088\u3073OBP-702\u306e\u6cbb\u7642\u52b9\u679c\u3092\u691c\u8a0e\u3057\u305f\u3002\u30a6\u30a4\u30eb\u30b9\u306b\u3088\u308b\u6297\u816b\u760d\u52b9\u679c\u306e\u5206\u5b50\u6a5f\u69cb\u3092\u660e\u3089\u304b\u306b\u3059\u308b\u305f\u3081\u306b\u3001\u30a6\u30a4\u30eb\u30b9\u611f\u67d3\u3057\u305fNB\u7d30\u80de\u306b\u304a\u3051\u308b\u30a2\u30dd\u30c8\u30fc\u30b7\u30b9\u304a\u3088\u3073\u30aa\u30fc\u30c8\u30d5\u30a1\u30b8\u30fc\u95a2\u9023\u30de\u30fc\u30ab\u30fc\u3068MYCN\u306e\u5909\u8abf\u3092\u8a55\u4fa1\u3057\u305f\u3002\u6700\u5f8c\u306b\u3001\u76ae\u4e0bNB\u7570\u7a2e\u79fb\u690d\u816b\u760d\u30e2\u30c7\u30eb\u3092\u7528\u3044\u3066\u3001\u30aa\u30f3\u30b3\u30ed\u30a4\u30c9\u6027\u30a2\u30c7\u30ce\u30a6\u30a4\u30eb\u30b9\u306ein vivo\u6297\u816b\u760d\u52b9\u679c\u3092\u8a55\u4fa1\u3057\u305f\u3002<\/p>\n<h2>\u7d50\u679c<\/h2>\n<p>MYCN\u5897\u5e45NB\u7d30\u80de\u306b\u304a\u3051\u308bCAR\u3068hTERT\u306e\u767a\u73fe<\/p>\n<p>\u30a2\u30c7\u30ce\u30a6\u30a4\u30eb\u30b9\u8840\u6e05\u578b 5\uff08Ad5\uff09\u306f\u3001\u30a6\u30a4\u30eb\u30b9\u7e4a\u7dad\u30ce\u30d6\u3068\u30b3\u30c3\u30af\u30b9\u30b5\u30c3\u30ad\u30fc\u30a6\u30a4\u30eb\u30b9\u304a\u3088\u3073\u30a2\u30c7\u30ce\u30a6\u30a4\u30eb\u30b9\u53d7\u5bb9\u4f53\uff08CAR\uff09\u30bf\u30f3\u30d1\u30af\u8cea\u3068\u306e\u7d50\u5408\u3092\u4ecb\u3057\u3066\u6a19\u7684\u7d30\u80de\u306b\u4fb5\u5165\u3059\u308b\u300217 Ad5 \u30b2\u30ce\u30e0\u306b\u57fa\u3065\u3044\u3066\u751f\u6210\u3055\u308c\u305f hTERT-driven oncolytic adenovirus \u306e NB \u7d30\u80de\u306b\u304a\u3051\u308b\u6cbb\u7642\u306e\u53ef\u80fd\u6027\u3092\u8a55\u4fa1\u3059\u308b\u305f\u3081\u306b\u3001\u6211\u3005\u306f\u30d5\u30ed\u30fc\u30b5\u30a4\u30c8\u30e1\u30c8\u30ea\u30fc\u5206\u6790\u3092\u7528\u3044\u3066\u30014 \u3064\u306e\u30d2\u30c8 MYCN \u5897\u5f37 NB \u7d30\u80de\u682a\uff08IMR-32\u3001CHP-134\u3001NB-1\u3001LA-N-5\uff09\u306b\u304a\u3051\u308b\u7d30\u80de\u8868\u9762 CAR \u30bf\u30f3\u30d1\u30af\u8cea\u306e\u767a\u73fe\u30ec\u30d9\u30eb\u3092\u6e2c\u5b9a\u3057\u307e\u3057\u305f\u3002\u3059\u3079\u3066\u306eNB\u7d30\u80de\u682a\u306f\u3001\u7d30\u80de\u8868\u9762\u306bCAR\u306e\u767a\u73fe\u3092\u793a\u3057\u305f\uff08\u56f31A\uff09\u3002\u6b21\u306b\u3001\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0RT-PCR\u5206\u6790\u3092\u7528\u3044\u3066\u3001MYCN\u5897\u5e45NB\u7d30\u80de\u306b\u304a\u3051\u308bhTERT mRNA\u306e\u767a\u73fe\u30ec\u30d9\u30eb\u3092\u6e2c\u5b9a\u3057\u305f\u3002\u30d2\u30c8\u80ba\u764cH1299\u7d30\u80de\u3068\u6bd4\u8f03\u3057\u3066\u3001\u3059\u3079\u3066\u306eNB\u7d30\u80de\u682a\u306f\u7d042\u500d\u304b\u308913\u500d\u306e\u9ad8\u3044hTERT mRNA\u306e\u767a\u73fe\u3092\u793a\u3057\u305f\uff08\u56f31B\uff09\u3002\u5bfe\u7167\u7684\u306b\u3001\u6b63\u5e38\u30d2\u30c8\u80ba\u7dda\u7dad\u82bd\u7d30\u80deWI38\u7d30\u80de\u3067\u306fhTERT mRNA\u306e\u767a\u73fe\u306f\u691c\u51fa\u3055\u308c\u306a\u304b\u3063\u305f\uff08\u56f31B\uff09\u3002\u3055\u3089\u306b\u3001\uff2d\uff39\uff23\uff2e\u5897\u5e45\uff2e\uff22\u7d30\u80de\u682a\u3067\u306f\u3001\u30a6\u30a8\u30b9\u30bf\u30f3\u30d6\u30ed\u30c3\u30c8\u306b\u3088\u308a\uff2d\uff39\uff23\uff2e\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u767a\u73fe\u3092\u78ba\u8a8d\u3057\u305f\uff08\u56f3\uff11\uff23\uff09\u3002\u89b3\u5bdf\u3055\u308c\u305f\uff23\uff21\uff32\u304a\u3088\u3073\uff48\uff34\uff25\uff32\uff34\u306e\u767a\u73fe\u306f\u3001\uff2d\uff39\uff23\uff2e\u5897\u5e45\uff2e\uff22\u7d30\u80de\u304c\uff48\uff34\uff25\uff32\uff34\u99c6\u52d5\u30aa\u30f3\u30b3\u30ed\u30a4\u30c9\u30a2\u30c7\u30ce\u30a6\u30a4\u30eb\u30b9\u306b\u654f\u611f\u3067\u3042\u308b\u3053\u3068\u3092\u793a\u5506\u3057\u3066\u3044\u308b\u3002<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-3200\" src=\"http:\/\/m12n08.jp\/blog\/wp-content\/uploads\/2020\/06\/2020-06-26_163856.jpg\" alt=\"\" width=\"715\" height=\"404\" srcset=\"http:\/\/m12n08.jp\/blog\/wp-content\/uploads\/2020\/06\/2020-06-26_163856.jpg 715w, http:\/\/m12n08.jp\/blog\/wp-content\/uploads\/2020\/06\/2020-06-26_163856-200x113.jpg 200w\" sizes=\"(max-width: 715px) 100vw, 715px\" \/><\/p>\n<p>\u56f31. MYCN\u5897\u5e45\u3092\u793a\u3059\u30d2\u30c8NB\u7d30\u80de\u306b\u304a\u3051\u308bCAR\u30bf\u30f3\u30d1\u30af\u8cea\u3068\u30d2\u30c8\u30c6\u30ed\u30e1\u30e9\u30fc\u30bc\u9006\u8ee2\u5199\u9175\u7d20\uff08hTERT\uff09mRNA\u306e\u767a\u73fe<\/p>\n<p>A\uff09\u30d2\u30c8NB\u7d30\u80de\u306b\u304a\u3051\u308bCAR\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u767a\u73fe\u3092\u30d5\u30ed\u30fc\u30b5\u30a4\u30c8\u30e1\u30c8\u30ea\u30fc\u3092\u7528\u3044\u3066\u89e3\u6790\u3057\u305f\u3002\u7d30\u80de\u3092\u30de\u30a6\u30b9\u6297CAR\u30e2\u30ce\u30af\u30ed\u30fc\u30ca\u30eb\u6297\u4f53\u3067\u30a4\u30f3\u30ad\u30e5\u30d9\u30fc\u30c8\u3057\u305f\u5f8c\u3001FITC\u6a19\u8b58\u4e8c\u6b21\u6297\u4f53\u3067\u691c\u51fa\u3057\u305f\u3002\u30a2\u30a4\u30bd\u30bf\u30a4\u30d7\u306b\u30de\u30c3\u30c1\u3057\u305f\u6b63\u5e38\u30de\u30a6\u30b9IgG\u3092\u30b3\u30f3\u30c8\u30ed\u30fc\u30eb\u3068\u3057\u3066\u4f7f\u7528\u3057\u305f\u3002(B) hTERT mRNA\u306e\u767a\u73fe\u3092qRT-PCR\u3092\u7528\u3044\u3066\u89e3\u6790\u3057\u305f\u3002hTERT mRNA\u306e\u767a\u73fe\u30ec\u30d9\u30eb\u306f\u3001H1299\u7d30\u80de\u306b\u304a\u3051\u308bhTERT mRNA\u306e\u767a\u73fe\u30ec\u30d9\u30eb\u30921\u3068\u3057\u305f\u3068\u304d\u306e\u76f8\u5bfe\u5024\u3092\u7b97\u51fa\u3057\u305f\u3002 \u30c7\u30fc\u30bf\u306f\u3001\u5e73\u5747\u00b1SD\uff08n = 3\uff09\u3068\u3057\u3066\u8868\u3057\u305f\u3002\uff23\uff09\u30d2\u30c8\uff2e\uff22\u7d30\u80de\u306b\u304a\u3051\u308b\uff2d\uff39\uff23\uff2e\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u767a\u73fe\u3092\u30a6\u30a8\u30b9\u30bf\u30f3\u30d6\u30ed\u30c3\u30c6\u30a3\u30f3\u30b0\u3092\u7528\u3044\u3066\u5206\u6790\u3057\u305f\u3002\u03b2-\u30a2\u30af\u30c1\u30f3\u3092\u30ed\u30fc\u30c7\u30a3\u30f3\u30b0\u30b3\u30f3\u30c8\u30ed\u30fc\u30eb\u3068\u3057\u3066\u30a2\u30c3\u30bb\u30a4\u3057\u305f\u3002<\/p>\n<h2>MYCN\u5897\u5e45NB\u7d30\u80de\u306b\u5bfe\u3059\u308bhTERT\u99c6\u52d5\u578b\u6297\u304c\u3093\u5264\u30a2\u30c7\u30ce\u30a6\u30a4\u30eb\u30b9\u306ein vitro\u7d30\u80de\u75c5\u7406\u5b66\u7684\u52b9\u679c<\/h2>\n<p>MYCN\u5897\u5e45NB\u7d30\u80de\u306b\u5bfe\u3059\u308bhTERT\u99c6\u52d5\u30aa\u30f3\u30b3\u30ed\u30a4\u30c9\u30a2\u30c7\u30ce\u30a6\u30a4\u30eb\u30b9\u306e\u6cbb\u7642\u53ef\u80fd\u6027\u3092\u8abf\u3079\u308b\u305f\u3081\u306b\u30013&#8242;-[1-(\u30d5\u30a7\u30cb\u30eb\u30a2\u30df\u30ce\u30ab\u30eb\u30dc\u30cb\u30eb)-3\u30ca\u30c8\u30ea\u30a6\u30e0\u3092\u7528\u3044\u3066\u3001\u30a6\u30a4\u30eb\u30b9\u611f\u67d3\u5f8c3\u65e5\u76ee\u306bNB\u7d30\u80de\u306e\u751f\u5b58\u7387\u3092\u8a55\u4fa1\u3057\u305f\u3002 4-\u30c6\u30c8\u30e9\u30be\u30ea\u30a6\u30e0]-\u30d3\u30b9\uff084-\u30e1\u30c8\u30ad\u30b7-6-\u30cb\u30c8\u30ed\uff09\u30d9\u30f3\u30bc\u30f3\u30b9\u30eb\u30db\u30f3\u9178\u6c34\u548c\u7269\uff082,3-\u30d3\u30b9-\uff082-\u30e1\u30c8\u30ad\u30b7-4-\u30cb\u30c8\u30ed-5-\u30b9\u30eb\u30db\u30d5\u30a7\u30cb\u30eb\uff092H-\u30c6\u30c8\u30e9\u30be\u30ea\u30a6\u30e0-5-\u30ab\u30eb\u30dc\u30ad\u30b5\u30cb\u30ea\u30c9\uff09\u30a2\u30c3\u30bb\u30a4\u3002\uff2f\uff22\uff30-\uff13\uff10\uff11\u304a\u3088\u3073\uff2f\uff22\uff30-\uff17\uff10\uff12\u306e\u4e21\u65b9\u3068\u3082\u3001\u7528\u91cf\u4f9d\u5b58\u7684\u306b\u3059\u3079\u3066\u306e\uff2e\uff22\u7d30\u80de\u306e\u751f\u5b58\u7387\u3092\u6709\u610f\u306b\u6e1b\u5c11\u3055\u305b\u305f\uff08\u56f3\uff12\uff21\uff1b\u56f3\uff33\uff11\uff09\u3002\uff29\uff2d\uff32-\uff13\uff12\u304a\u3088\u3073\uff2c\uff21-\uff2e-\uff15\u7d30\u80de\u306e\u751f\u5b58\u7387\u306f\u3001\u30a6\u30a4\u30eb\u30b9\u611f\u67d3\u5f8c\uff15\u65e5\u76ee\u306b\u3055\u3089\u306b\u4f4e\u4e0b\u3057\u305f\uff08\u56f3\uff33\uff12\uff09\u3002OBP-301\u3068\u6bd4\u8f03\u3057\u3066\u3001OBP-702\u306f\u3001\u691c\u67fb\u3057\u305f\u3059\u3079\u3066\u306eNB\u7d30\u80de\u682a\u306b\u5bfe\u3057\u3066\u3088\u308a\u5f37\u3044\u6297\u816b\u760d\u52b9\u679c\u3092\u793a\u3057\u305f\u3002\u307e\u305f\u3001SK-N-SH \u7d30\u80de\u306f\u975eMYCN\u5897\u5e45\u578bNB \u7d30\u80de\u3068\u3057\u3066\u5831\u544a\u3055\u308c\u3066\u304a\u308a\u3001hTERT \u306e\u767a\u73fe\u304c\u9ad8\u3044\u3053\u3068\u304c\u5831\u544a\u3055\u308c\u3066\u3044\u308b18 \u3002\u975eMYCN\u5897\u5e45SK-N-SH\u7d30\u80de\u306f\u9ad8\u3044hTERT\u767a\u73fe\u3092\u793a\u3057\u3001OBP-301\u304a\u3088\u3073OBP-702\u306b\u5bfe\u3057\u3066\u3082MYCN\u5897\u5e45NB\u7d30\u80de\u3068\u540c\u69d8\u306b\u611f\u53d7\u6027\u3092\u793a\u3057\u305f\uff08\u56f3S3\u304a\u3088\u3073S4A\uff09\u3002\u3053\u308c\u3089\u306e\u7d50\u679c\u306f\u3001hTERT\u3092\u767a\u73fe\u3059\u308bNB\u7d30\u80de\u304cOBP-301\u304a\u3088\u3073OBP-702\u306b\u654f\u611f\u3067\u3042\u308b\u3053\u3068\u3092\u793a\u5506\u3057\u3066\u3044\u308b\u3002<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-3202\" src=\"http:\/\/m12n08.jp\/blog\/wp-content\/uploads\/2020\/06\/2020-06-26_164148.jpg\" alt=\"\" width=\"756\" height=\"481\" srcset=\"http:\/\/m12n08.jp\/blog\/wp-content\/uploads\/2020\/06\/2020-06-26_164148.jpg 756w, http:\/\/m12n08.jp\/blog\/wp-content\/uploads\/2020\/06\/2020-06-26_164148-189x120.jpg 189w\" sizes=\"(max-width: 706px) 89vw, (max-width: 767px) 82vw, 740px\" \/><\/p>\n<p>\u56f32. \u30d2\u30c8NB\u7d30\u80de\u306b\u304a\u3051\u308b\u30aa\u30fc\u30c8\u30d5\u30a1\u30b8\u30fc\u3068\u306e\u95a2\u9023\u306b\u304a\u3051\u308bOBP-301\u304a\u3088\u3073OBP-702\u306ein vitro\u7d30\u80de\u75c5\u7406\u5b66\u7684\u52b9\u679c<\/p>\n<p><span style=\"font-size: 10pt;\">\uff21\uff09\uff29\uff2d\uff32-\uff13\uff12\u304a\u3088\u3073\uff23\uff28\uff30-\uff11\uff13\uff14\u7d30\u80de\u3092\u6307\u793a\u3055\u308c\u305f\uff2d\uff2f\uff29\u3067\uff2f\uff22\uff30-\uff13\uff10\uff11\u307e\u305f\u306f\uff2f\uff22\uff30-\uff17\uff10\uff12\u306b\u611f\u67d3\u3055\u305b\u3001\u611f\u67d3\u5f8c\uff13\u65e5\u76ee\u306b\uff38\uff34\uff34\u30a2\u30c3\u30bb\u30a4\u3092\u7528\u3044\u3066\u7d30\u80de\u751f\u5b58\u7387\u3092\u8a55\u4fa1\u3057\u305f\u3002\u7d30\u80de\u751f\u5b58\u7387\u306f\u30011.0\u3068\u3057\u305f\u30e2\u30c3\u30af\u611f\u67d3\u7d30\u80de\u306b\u5bfe\u3059\u308b\u76f8\u5bfe\u5024\u3092\u7b97\u51fa\u3057\u305f\u3002\u7d30\u80de\u751f\u5b58\u7387\u30c7\u30fc\u30bf\u306f\u3001\u5e73\u5747\u5024\u00b1SD\uff08n = 5\uff09\u3067\u8868\u3057\u305f\u3002\u2217p &lt; 0.05\uff08\u5bfe MOI 0\uff09\u3002(B) OBP-301\u307e\u305f\u306fOBP-702\u309272\u6642\u9593\u6295\u4e0e\u3057\u305fIMR-32\u304a\u3088\u3073CHP-134\u7d30\u80de\u306b\u304a\u3051\u308b\u30a6\u30a4\u30eb\u30b9E1A\u3001p53\u3001PARP\u3001\u958b\u88c2PARP\uff08C-PARP\uff09\u304a\u3088\u3073\u5fae\u5c0f\u7ba1\u95a2\u9023\u30bf\u30f3\u30d1\u30af\u8cea1\u8efd\u93963\uff08LC3\uff09\u306e\u767a\u73fe\u3002\u30d2\u30c8NB\u7d30\u80de\u306b\u304a\u3051\u308b\u30aa\u30fc\u30c8\u30d5\u30a1\u30b8\u30fc\u3068\u306e\u95a2\u9023\u3067\u306eOBP-301\u304a\u3088\u3073OBP-702\u306ein vitro\u7d30\u80de\u75c5\u7406\u5b66\u7684\u52b9\u679c<\/span><\/p>\n<p><span style=\"font-size: 10pt;\">\uff21\uff09\uff29\uff2d\uff32-\uff13\uff12\u304a\u3088\u3073\uff23\uff28\uff30-\uff11\uff13\uff14\u7d30\u80de\u3092\u6307\u793a\u3055\u308c\u305f\uff2d\uff2f\uff29\u3067\uff2f\uff22\uff30-\uff13\uff10\uff11\u307e\u305f\u306f\uff2f\uff22\uff30-\uff17\uff10\uff12\u306b\u611f\u67d3\u3055\u305b\u3001\u611f\u67d3\u5f8c\uff13\u65e5\u76ee\u306b\uff38\uff34\uff34\u30a2\u30c3\u30bb\u30a4\u3092\u7528\u3044\u3066\u7d30\u80de\u751f\u5b58\u7387\u3092\u8a55\u4fa1\u3057\u305f\u3002\u7d30\u80de\u751f\u5b58\u7387\u306f\u30011.0\u3068\u3057\u305f\u30e2\u30c3\u30af\u611f\u67d3\u7d30\u80de\u306b\u5bfe\u3059\u308b\u76f8\u5bfe\u5024\u3092\u7b97\u51fa\u3057\u305f\u3002\u7d30\u80de\u751f\u5b58\u7387\u30c7\u30fc\u30bf\u306f\u3001\u5e73\u5747\u5024\u00b1SD\uff08n = 5\uff09\u3067\u8868\u3057\u305f\u3002\u2217p &lt; 0.05\uff08\u5bfe MOI 0\uff09\u3002(B) OBP-301\u307e\u305f\u306fOBP-702\u309272\u6642\u9593\u6295\u4e0e\u3057\u305fIMR-32\u304a\u3088\u3073CHP-134\u7d30\u80de\u306b\u304a\u3051\u308b\u30a6\u30a4\u30eb\u30b9E1A\u3001p53\u3001PARP\u3001\u958b\u88c2PARP\uff08C-PARP\uff09\u3001\u304a\u3088\u3073\u5fae\u5c0f\u7ba1\u95a2\u9023\u30bf\u30f3\u30d1\u30af\u8cea1\u8efd\u93963\uff08LC3\uff09\u306e\u767a\u73fe\u3002<\/span><\/p>\n<hr \/>\n<p>MYCN \u5897\u6b96\u3057\u305fNB\u7d30\u80de\u306b\u5bfe\u3059\u308b\u30a6\u30a4\u30eb\u30b9\u5a92\u4ecb\u6297\u816b\u760d\u52b9\u679c\u306e\u6839\u5e95\u306b\u3042\u308b\u30e1\u30ab\u30cb\u30ba\u30e0\u3092\u63a2\u308b\u305f\u3081\u306b\u3001\u30a6\u30a4\u30eb\u30b9\u611f\u67d3\u5f8c3\u65e5\u76ee\u306e\u30a2\u30dd\u30c8\u30fc\u30b7\u30b9\u95a2\u9023\u30bf\u30f3\u30d1\u30af\u8cea\u304a\u3088\u3073\u30aa\u30fc\u30c8\u30d5\u30a1\u30b8\u30fc\u95a2\u9023\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u767a\u73fe\u3092\u30a6\u30a8\u30b9\u30bf\u30f3\u30d6\u30ed\u30c3\u30c8\u89e3\u6790\u3092\u7528\u3044\u3066\u8abf\u3079\u305f\u3002\u305d\u306e\u7d50\u679c\u3001\u30a2\u30dd\u30c8\u30fc\u30b7\u30b9\u95a2\u9023\u30de\u30fc\u30ab\u30fc\u3067\u3042\u308b\u5207\u65ad\u30dd\u30ea\uff08ADP-\u30ea\u30dc\u30fc\u30b9\uff09\u30dd\u30ea\u30e1\u30e9\u30fc\u30bc\uff08PARP\uff09\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u767a\u73fe\u5897\u52a0\u306f\u3001OBP-301\u304a\u3088\u3073OBP-702\u611f\u67d3\u5f8c\u306b\u306f\u8a8d\u3081\u3089\u308c\u306a\u304b\u3063\u305f\uff08\u56f32B\uff09\u3002\u4e00\u65b9\u3001OBP-301\u304a\u3088\u3073OBP-702\u306e\u3044\u305a\u308c\u3082\u3001\u30aa\u30fc\u30c8\u30d5\u30a1\u30b8\u30fc\u8a98\u5c0e\u6642\u306bLC3-I\u30bf\u30f3\u30d1\u30af\u8cea\u304b\u3089\u5909\u63db\u3055\u308c\u308b\u30aa\u30fc\u30c8\u30d5\u30a1\u30b8\u30fc\u95a2\u9023\u30de\u30fc\u30ab\u30fcLC3-II\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u767a\u73fe\u5897\u52a0\u3092\u8a98\u5c0e\u3057\u305f\u3002\u3057\u304b\u3057\u3001NB\u7d30\u80de\u3067\u306fp62\u306e\u767a\u73fe\u306f\u691c\u51fa\u3055\u308c\u306a\u304b\u3063\u305f\uff08\u30c7\u30fc\u30bf\u306f\u793a\u3057\u3066\u3044\u306a\u3044\uff09\u3002\u30a2\u30c7\u30ce\u30a6\u30a4\u30eb\u30b9E1A\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u767a\u73fe\u306f\u3001OBP-301\u307e\u305f\u306fOBP-702\u306e\u3044\u305a\u308c\u304b\u306b\u611f\u67d3\u3057\u305f\u5168\u3066\u306eNB\u7d30\u80de\u3067\u5897\u52a0\u3057\u305f\u304c\u3001p53\u306e\u767a\u73fe\u306fOBP-301\u3067\u306f\u6e1b\u5c11\u3057\u3001OBP-702\u3067\u306f\u5897\u52a0\u3057\u305f\u3002\u4e00\u65b9\u3001\u975eMYCN\u5897\u5e45NB\u7d30\u80de\u306f\u3001\u30a6\u30a4\u30eb\u30b9\u611f\u67d3\u5f8c\u306b\u30a2\u30dd\u30c8\u30fc\u30b7\u30b9\u3068\u30aa\u30fc\u30c8\u30d5\u30a1\u30b8\u30fc\u3092\u793a\u3057\u305f\uff08\u56f3S4B\uff09\u3002\u3053\u308c\u3089\u306e\u7d50\u679c\u306f\u3001OBP-301\u304a\u3088\u3073OBP-702\u306e\u4e21\u65b9\u306e\u6297\u816b\u760d\u52b9\u679c\u304c\u3001MYCN\u5897\u5e45NB\u7d30\u80de\u306b\u304a\u3051\u308b\u30aa\u30fc\u30c8\u30d5\u30a1\u30b8\u30fc\u306e\u8a98\u5c0e\u3068\u95a2\u9023\u3057\u3066\u3044\u308b\u3053\u3068\u3092\u793a\u5506\u3057\u3066\u3044\u308b\u3002<\/p>\n<h2>hTERT\u99c6\u52d5\u578b\u6297\u304c\u3093\u5264\u30a2\u30c7\u30ce\u30a6\u30a4\u30eb\u30b9\u306fMYCN\u306e\u767a\u73fe\u3092\u4f4e\u4e0b\u3055\u305b\u308b<\/h2>\n<p>MYCN\u306f\u3001MYCN\u5897\u5e45NB\u7d30\u80de\u306e\u60aa\u6027\u5ea6\u7dad\u6301\u306b\u91cd\u8981\u306a\u5f79\u5272\u3092\u679c\u305f\u3057\u3066\u3044\u308b\u3002\u305d\u3053\u3067\u3001OBP-301\u307e\u305f\u306fOBP-702\u3092\u611f\u67d3\u3055\u305b\u305fMYCN\u5897\u5e45NB\u7d30\u80de\u306b\u304a\u3051\u308bMYCN mRNA\u304a\u3088\u3073\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u767a\u73fe\u30ec\u30d9\u30eb\u3092\u8a55\u4fa1\u3057\u305f\u3002MYCN mRNA\u304a\u3088\u3073\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u4e21\u65b9\u306e\u767a\u73fe\u306f\u3001OBP-301\u304a\u3088\u3073OBP-702\u306b\u611f\u67d3\u3057\u305fNB\u7d30\u80de\u306b\u304a\u3044\u3066\u3001\u7528\u91cf\u4f9d\u5b58\u7684\u306b\u30c0\u30a6\u30f3\u30ec\u30ae\u30e5\u30ec\u30fc\u30c8\u3055\u308c\u305f\uff08\u56f33A\u304a\u3088\u30733B\uff09\u3002\u30a6\u30a4\u30eb\u30b9\u8a98\u767a\u6027MYCN\u30c0\u30a6\u30f3\u30ec\u30ae\u30e5\u30ec\u30fc\u30b7\u30e7\u30f3\u306e\u57fa\u790e\u3068\u306a\u308b\u30e1\u30ab\u30cb\u30ba\u30e0\u3092\u63a2\u308b\u305f\u3081\u306b\u3001\u30a6\u30a4\u30eb\u30b9E1A\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u4e0b\u6d41\u30e1\u30c7\u30a3\u30a8\u30fc\u30bf\u30fc\u3067\u3042\u308bE2F1\u306e\u5f79\u5272\u3092\u8abf\u3079\u305f\u3002E2F1\u306e\u767a\u73fe\u306f\u3001OBP-301\u304a\u3088\u3073OBP-702\u306b\u611f\u67d3\u3057\u305fNB\u7d30\u80de\u306b\u304a\u3044\u3066\u3001\u7528\u91cf\u4f9d\u5b58\u7684\u306b\u30a2\u30c3\u30d7\u30ec\u30ae\u30e5\u30ec\u30fc\u30c8\u3055\u308c\u305f\uff08\u56f33B\uff09\u3002OBP-301\u304a\u3088\u3073OBP-702\u611f\u67d3NB\u816b\u760d\u7d30\u80de\u306b\u304a\u3044\u3066\u3001E2F1\u3068MYCN mRNA\u304a\u3088\u3073\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u767a\u73fe\u306e\u9593\u306b\u6709\u610f\u306a\u9006\u76f8\u95a2\u304c\u89b3\u5bdf\u3055\u308c\u305f\uff08\u56f33C\uff1b\u56f3S5\uff09\u3002\u3053\u308c\u3089\u306e\u7d50\u679c\u306f\u3001E2F1\u304c\u30a6\u30a4\u30eb\u30b9\u5a92\u4ecb\u306b\u3088\u308bMYCN\u767a\u73fe\u306e\u30c0\u30a6\u30f3\u30ec\u30ae\u30e5\u30ec\u30fc\u30b7\u30e7\u30f3\u306b\u5f79\u5272\u3092\u679c\u305f\u3057\u3066\u3044\u308b\u3053\u3068\u3092\u793a\u5506\u3057\u3066\u3044\u308b\u3002<\/p>\n<hr \/>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-3203\" src=\"http:\/\/m12n08.jp\/blog\/wp-content\/uploads\/2020\/06\/2020-06-26_164441.jpg\" alt=\"\" width=\"744\" height=\"558\" srcset=\"http:\/\/m12n08.jp\/blog\/wp-content\/uploads\/2020\/06\/2020-06-26_164441.jpg 744w, http:\/\/m12n08.jp\/blog\/wp-content\/uploads\/2020\/06\/2020-06-26_164441-160x120.jpg 160w\" sizes=\"(max-width: 706px) 89vw, (max-width: 767px) 82vw, 740px\" \/><\/p>\n<p><span style=\"font-size: 10pt;\">\u56f33. OBP-301\u304a\u3088\u3073OBP-702\u306fE2F1\u306e\u30a2\u30c3\u30d7\u30ec\u30ae\u30e5\u30ec\u30fc\u30b7\u30e7\u30f3\u3068\u95a2\u9023\u3057\u3066MYCN\u306e\u30c0\u30a6\u30f3\u30ec\u30ae\u30e5\u30ec\u30fc\u30b7\u30e7\u30f3\u3092\u8a98\u5c0e\u3059\u308b<\/span><\/p>\n<p><span style=\"font-size: 10pt;\">\uff21\uff09\u6307\u793a\u3055\u308c\u305f\uff2d\uff2f\uff29\u3067\uff2f\uff22\uff30-\uff13\uff10\uff11\u307e\u305f\u306f\uff2f\uff22\uff30-\uff17\uff10\uff12\u3092\u611f\u67d3\u3055\u305b\u305f\uff29\uff2d\uff32-\uff13\uff12\u304a\u3088\u3073\uff23\uff28\uff30-\uff11\uff13\uff14\u7d30\u80de\u306b\u304a\u3051\u308b\uff2d\uff39\uff23\uff2e \uff4d\uff32\uff2e\uff21\u306e\u767a\u73fe\u3092\uff17\uff12\u6642\u9593\u3068\u3057\u3001\u30e2\u30c3\u30af\u611f\u67d3\u7d30\u80de\u306b\u304a\u3051\u308b\uff2d\uff39\uff23\uff2e \uff4d\uff32\uff2e\uff21\u306e\u5024\u3092\uff11\u3068\u3057\u3001\u611f\u67d3\u7d30\u80de\u306b\u304a\u3051\u308b\uff2d\uff39\uff23\uff2e \uff4d\uff32\uff2e\uff21\u306e\u76f8\u5bfe\u7684\u306a\u30ec\u30d9\u30eb\u3092\u500d\u6570\u8a98\u5c0e\u3068\u3057\u3066\u30d7\u30ed\u30c3\u30c8\u3057\u305f\u3002\u30c7\u30fc\u30bf\u306f\u5e73\u5747\u5024\u00b1SD\uff08n = 3\uff09\u3067\u8868\u3057\u305f\u3002\u2217p\uff1c0.05\uff08\u5bfeMOI\u306f0\uff09\u3002(B) OBP-301\u307e\u305f\u306fOBP-702\u3092\u6307\u793a\u3055\u308c\u305fMOI\u306772\u6642\u9593\u611f\u67d3\u3055\u305b\u305fIMR-32\u304a\u3088\u3073CHP-134\u7d30\u80de\u306b\u304a\u3051\u308bMYCN\u304a\u3088\u3073E2F1\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u767a\u73fe\u3002MYCN\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u767a\u73fe\u30ec\u30d9\u30eb\u306f\u30011.0\u3068\u3057\u305f\u30e2\u30c3\u30af\u611f\u67d3\u7d30\u80de\u306e\u767a\u73fe\u30ec\u30d9\u30eb\u3068\u306e\u76f8\u5bfe\u5024\u3092\u8a08\u7b97\u3057\u305f\u3002E2F1\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u767a\u73fe\u91cf\u306f\u30011.0\u306b\u8a2d\u5b9a\u3057\u305f\u6700\u3082E2F1\u6d3b\u6027\u306e\u9ad8\u3044\u7d30\u80de\u306e\u767a\u73fe\u91cf\u3068\u76f8\u5bfe\u7684\u306b\u8a08\u7b97\u3057\u305f\u3002\uff23\uff09\uff2f\uff22\uff30-\uff13\uff10\uff11\u307e\u305f\u306f\uff2f\uff22\uff30-\uff17\uff10\uff12\u3067\u611f\u67d3\u3055\u305b\u305f\uff29\uff2d\uff32-\uff13\uff12\u304a\u3088\u3073\uff23\uff28\uff30-\uff11\uff13\uff14\u7d30\u80de\u306b\u304a\u3051\u308b\uff25\uff12\uff26\uff11\u304a\u3088\u3073\uff2d\uff39\uff23\uff2e\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u767a\u73fe\u91cf\u306e\u76f8\u95a2\u3002<\/span><\/p>\n<hr \/>\n<h2>E2F1\u306f\u6297\u304c\u3093\u5264\u30a2\u30c7\u30ce\u30a6\u30a4\u30eb\u30b9\u8a98\u5c0eMYCN\u6291\u5236\u306b\u95a2\u4e0e\u3057\u3066\u3044\u308b<\/h2>\n<p>E2F1\u3092\u767a\u73fe\u3059\u308b\u975e\u8907\u88fd\u30a2\u30c7\u30ce\u30a6\u30a4\u30eb\u30b9Ad-E2F1\u3092\u7528\u3044\u3066\u3001MYCN\u3068E2F1\u306e\u95a2\u4fc2\u3092\u8abf\u3079\u305f\u3068\u3053\u308d\u3001E2F1\u306e\u7570\u6240\u6027\u767a\u73fe\u306fIMR-32\u7d30\u80de\u3068CHP-134\u7d30\u80de\u306b\u304a\u3044\u3066MYCN\u306emRNA\u3068\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u767a\u73fe\u3092\u4f4e\u4e0b\u3055\u305b\u305f\uff08\u56f34A-4C\uff09\u3002E2F1\u30b9\u30e2\u30fc\u30eb\u30a4\u30f3\u30bf\u30fc\u30d5\u30a7\u30ea\u30f3\u30b0RNA\uff08siRNA\uff09\u306b\u3088\u308b\u524d\u51e6\u7406\u306f\u3001E2F1\u306e\u6291\u5236\u3092\u4ecb\u3057\u3066Ad-E2F1\u611f\u67d3NB\u7d30\u80de\u306b\u304a\u3051\u308bMYCN\u30c0\u30a6\u30f3\u30ec\u30ae\u30e5\u30ec\u30fc\u30b7\u30e7\u30f3\u3092\u6e1b\u8870\u3055\u305b\u305f\uff08\u56f34D\uff09\u3002\u5bfe\u7167\u7684\u306b\u3001E1A\u6b20\u5931\u30b3\u30f3\u30c8\u30ed\u30fc\u30eb\u30a2\u30c7\u30ce\u30a6\u30a4\u30eb\u30b9dl312\u306b\u611f\u67d3\u3057\u305fNB\u7d30\u80de\u3067\u306f\u3001MYCN\u30bf\u30f3\u30d1\u30af\u8cea\u767a\u73fe\u306e\u6291\u5236\u306f\u89b3\u5bdf\u3055\u308c\u306a\u304b\u3063\u305f\uff08\u56f3S6\uff09\u3002\u3053\u308c\u3089\u306e\u7d50\u679c\u306f\u3001\u30a6\u30a4\u30eb\u30b9\u5a92\u4ecb\u306eE2F1\u6d3b\u6027\u5316\u304cMYCN\u5897\u5e45NB\u7d30\u80de\u306b\u304a\u3051\u308bMYCN\u767a\u73fe\u3092\u30c0\u30a6\u30f3\u30ec\u30ae\u30e5\u30ec\u30fc\u30c8\u3059\u308b\u3053\u3068\u3092\u793a\u5506\u3057\u3066\u3044\u308b\u3002<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-3204\" src=\"http:\/\/m12n08.jp\/blog\/wp-content\/uploads\/2020\/06\/2020-06-26_164637.jpg\" alt=\"\" width=\"620\" height=\"404\" srcset=\"http:\/\/m12n08.jp\/blog\/wp-content\/uploads\/2020\/06\/2020-06-26_164637.jpg 620w, http:\/\/m12n08.jp\/blog\/wp-content\/uploads\/2020\/06\/2020-06-26_164637-184x120.jpg 184w\" sizes=\"(max-width: 620px) 100vw, 620px\" \/><\/p>\n<p>\u56f34. E2F1\u306e\u6d3b\u6027\u5316\u306fMYCN\u306e\u30c0\u30a6\u30f3\u30ec\u30ae\u30e5\u30ec\u30fc\u30b7\u30e7\u30f3\u3092\u8a98\u5c0e\u3059\u308b<\/p>\n<p><span style=\"font-size: 10pt;\">(A) Ad-E2F1 \u3092\u6295\u4e0e\u3057\u305f IMR-32 \u7d30\u80de\u304a\u3088\u3073 CHP-134 \u7d30\u80de\u306b\u304a\u3051\u308b E2F1 \u30bf\u30f3\u30d1\u30af\u8cea\u306e 72 \u6642\u9593\u767a\u73fe (B) Ad-E2F1 \u3092\u6295\u4e0e\u3057\u305f IMR-32 \u7d30\u80de\u304a\u3088\u3073 CHP-134 \u7d30\u80de\u306b\u304a\u3051\u308b MYCN mRNA \u306e 72 \u6642\u9593\u767a\u73fe (MOI) \u3092\u793a\u3057\u305f\u3002\u30c7\u30fc\u30bf\u306f\u3001\u5e73\u5747\u00b1SD\uff08n = 3; *p &lt; 0.05 [\u5bfe MOI 0]\uff09\u3068\u3057\u3066\u8868\u3055\u308c\u308b\u3002(D) \u30b3\u30f3\u30c8\u30ed\u30fc\u30eb siRNA \u307e\u305f\u306f E2F1 siRNA (100 nM)\u3067\u524d\u51e6\u7406\u3057\u305f IMR-32 \u7d30\u80de\u304a\u3088\u3073 CHP-134 \u7d30\u80de\u306b\u304a\u3051\u308b MYCN \u304a\u3088\u3073 E2F1 \u30bf\u30f3\u30d1\u30af\u8cea\u306e\u767a\u73fe (100 MOI)\u3002<\/span><\/p>\n<hr \/>\n<h2>OBP-702\u3092\u4ecb\u3057\u305fp53\u6d3b\u6027\u5316\u306f\u30a6\u30a4\u30eb\u30b9\u611f\u67d3\u5f8c\u671f\u306b\u6e1b\u8870\u3059\u308b<\/h2>\n<p>\u6211\u3005\u306f\u3001OBP-702 \u304c p53 \u6d3b\u6027\u5316\u3092\u4ecb\u3057\u3066\u69d8\u3005\u306a\u30bf\u30a4\u30d7\u306e\u816b\u760d\u7d30\u80de\u306b\u304a\u3044\u3066 OBP-301 \u3088\u308a\u3082\u6df1\u3044\u6297\u816b\u760d\u52b9\u679c\u3092\u8a98\u5c0e\u3059\u308b\u3053\u3068\u3092\u5b9f\u8a3c\u3057\u305f11,14\u3002OBP-301 \u307e\u305f\u306f OBP-702 \u611f\u67d3\u5f8c 0\u300124\u300148\u300172 \u6642\u9593\u5f8c\u306e IMR-32 \u304a\u3088\u3073 CHP-134 \u7d30\u80de\u304b\u3089\u30bf\u30f3\u30d1\u30af\u8cea\u30e9\u30a4\u30bb\u30fc\u30c8\u3092\u8abf\u88fd\u3057\u305f\u3002\u30a2\u30c7\u30ce\u30a6\u30a4\u30eb\u30b9 E1A \u306e\u767a\u73fe\u306f 24-48 h \u3067\u5897\u52a0\u3057\u3001\u4e0b\u6d41\u30e1\u30c7\u30a3\u30a8\u30fc\u30bf\u30fc E2F1 \u306e\u767a\u73fe\u306f OBP-301 \u307e\u305f\u306f OBP-702 \u611f\u67d3\u5f8c 48-72 h \u3067\u5897\u52a0\u3057\u305f\uff08\u56f3 5\uff09\u3002OBP-301 \u306b\u611f\u67d3\u3057\u305f NB \u7d30\u80de\u3067\u306f\u300148 \u6642\u9593\u5f8c\u306b MYCN \u767a\u73fe\u304c\u6e1b\u5c11\u3057\u3001E2F1 \u306e\u30a2\u30c3\u30d7\u30ec\u30ae\u30e5\u30ec\u30fc\u30b7\u30e7\u30f3\u3068\u4e00\u81f4\u3057\u305f\u3002\u4e00\u65b9\u3001OBP-702\u611f\u67d3NB\u7d30\u80de\u3067\u306f\u3001p53\u306e\u767a\u73fe\u306f\u4f4e\u4e0b\u3057\u305f\u3082\u306e\u306e\u300124\u6642\u9593\u5f8c\u306b\u306fMYCN\u306e\u767a\u73fe\u304c\u4f4e\u4e0b\u3057\u3001E2F1\u306e\u767a\u73fe\u306f\u4f4e\u4e0b\u3057\u305f\u3082\u306e\u306e\u300148\uff5e72\u6642\u9593\u5f8c\u306b\u306fMYCN\u306e\u6291\u5236\u304c\u5897\u5f37\u3055\u308c\u305f\u3002\u3053\u308c\u3089\u306e\u7d50\u679c\u306f\u3001OBP-702\u3092\u4ecb\u3057\u305fMYCN\u30c0\u30a6\u30f3\u30ec\u30ae\u30e5\u30ec\u30fc\u30b7\u30e7\u30f3\u306b\u306f\u3001p53\u3067\u306f\u306a\u304fE2F1\u304c\u4e3b\u306b\u95a2\u4e0e\u3057\u3066\u3044\u308b\u3053\u3068\u3092\u793a\u5506\u3057\u3066\u3044\u308b\u3002<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-3205\" src=\"http:\/\/m12n08.jp\/blog\/wp-content\/uploads\/2020\/06\/2020-06-26_164840.jpg\" alt=\"\" width=\"781\" height=\"328\" srcset=\"http:\/\/m12n08.jp\/blog\/wp-content\/uploads\/2020\/06\/2020-06-26_164840.jpg 781w, http:\/\/m12n08.jp\/blog\/wp-content\/uploads\/2020\/06\/2020-06-26_164840-200x84.jpg 200w, http:\/\/m12n08.jp\/blog\/wp-content\/uploads\/2020\/06\/2020-06-26_164840-768x323.jpg 768w\" sizes=\"(max-width: 767px) 89vw, (max-width: 1000px) 54vw, (max-width: 1071px) 543px, 580px\" \/><\/p>\n<p>\u56f35. OBP-702\u3092\u4ecb\u3057\u305fp53\u306e\u6d3b\u6027\u5316\u306fMYCN\u306e\u30c0\u30a6\u30f3\u30ec\u30ae\u30e5\u30ec\u30fc\u30b7\u30e7\u30f3\u306b\u306f\u95a2\u4e0e\u3057\u3066\u3044\u306a\u3044<\/p>\n<p>IMR-32 \u304a\u3088\u3073 CHP-134 \u7d30\u80de\u3092 MOI 100 \u3067 OBP-301 \u307e\u305f\u306f OBP-702 \u306b\u611f\u67d3\u3055\u305b\u3001\u6307\u793a\u3055\u308c\u305f\u6642\u70b9\u3067\u7d30\u80de\u3092\u63a1\u53d6\u3057\u305f\u3002\u7d30\u80de\u6eb6\u89e3\u7269\u4e2d\u306eE1A\u3001p53\u3001PARP\u3001C-PARP\u3001LC3-I\u3001LC3-II\u3001E2F1\u3001\u304a\u3088\u3073MYCN\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u30ec\u30d9\u30eb\u3092\u30a6\u30a8\u30b9\u30bf\u30f3\u30d6\u30ed\u30c3\u30c8\u6cd5\u3067\u5206\u6790\u3057\u305f\u3002\u03b2-\u30a2\u30af\u30c1\u30f3\u3092\u30ed\u30fc\u30c7\u30a3\u30f3\u30b0\u30b3\u30f3\u30c8\u30ed\u30fc\u30eb\u3068\u3057\u3066\u30a2\u30c3\u30bb\u30a4\u3057\u305f\u3002<\/p>\n<hr \/>\n<h2>\u76ae\u4e0bCHP-134\u30bc\u30ce\u30b0\u30e9\u30d5\u30c8\u816b\u760d\u30e2\u30c7\u30eb\u306b\u304a\u3051\u308bhTERT-Driven Oncolytic Adenovirus\u306e\u30a4\u30f3\u30d3\u30dc\u3067\u306e\u6297\u816b\u760d\u52b9\u679c<\/h2>\n<p>\u6700\u5f8c\u306b\u3001hTERT\u99c6\u52d5\u30aa\u30f3\u30b3\u30ea\u30fc\u5316\u30a2\u30c7\u30ce\u30a6\u30a4\u30eb\u30b9\u306ein vivo\u6297\u816b\u760d\u52b9\u679c\u3092\u8a55\u4fa1\u3059\u308b\u305f\u3081\u306b\u3001\u76ae\u4e0bCHP-134\u30ad\u30bb\u30ce\u30b0\u30e9\u30d5\u30c8\u816b\u760d\u30e2\u30c7\u30eb\u3092\u4f7f\u7528\u3057\u305f\u3002\u30a6\u30a4\u30eb\u30b9\u51e6\u7406\u306e\u7570\u306a\u308b\u30d7\u30ed\u30c8\u30b3\u30eb\u3092\u7528\u3044\u30662\u3064\u306ein vivo\u5b9f\u9a13\u3092\u884c\u3063\u305f\u3002\uff2f\uff22\uff30-\uff13\uff10\uff11\u3001\uff2f\uff22\uff30-\uff17\uff10\uff12\u3001\u307e\u305f\u306f\uff30\uff22\uff33\u3092\u3001\u9031\u306b\uff11\u56de\u3001\u307e\u305f\u306f\u9031\u306b\uff13\u56de\u3001\uff13\u30b5\u30a4\u30af\u30eb\u306e\u9593\u3001\u76ae\u5185\u306b\u6ce8\u5165\u3057\u305f\u3002\u30a6\u30a4\u30eb\u30b9\u306e\uff13\u56de\u306e\u6295\u4e0e\u3067\u306f\u816b\u760d\u5897\u6b96\u3092\u6291\u5236\u3059\u308b\u306e\u306b\u5341\u5206\u3067\u306f\u306a\u304b\u3063\u305f\u304c\uff08\u56f3\uff33\uff17\uff09\u3001\uff23\uff28\uff30-\uff11\uff13\uff14\u816b\u760d\u7570\u7a2e\u79fb\u690d\u30e2\u30c7\u30eb\u306b\u304a\u3044\u3066\u3001\uff2f\uff22\uff30-\uff13\uff10\uff11\u307e\u305f\u306f\uff2f\uff22\uff30-\uff17\uff10\uff12\u306e\uff19\u56de\u306e\u6295\u4e0e\u306f\u3001\uff30\uff22\uff33\u3068\u6bd4\u8f03\u3057\u3066\u816b\u760d\u5897\u6b96\u3092\u6709\u610f\u306b\u6291\u5236\u3057\u305f\uff08\u56f3\uff16\uff21\u304a\u3088\u3073\uff16\uff22\uff09\u3002\u3057\u304b\u3057\u3001\u816b\u760d\u4f53\u7a4d\u306b\u306f\u3001OBP-301\u3068OBP-702\u6cbb\u7642\u306e\u9593\u306b\u7d71\u8a08\u7684\u306b\u6709\u610f\u306a\u5dee\u306f\u8a8d\u3081\u3089\u308c\u306a\u304b\u3063\u305f\u3002\u514d\u75ab\u7d44\u7e54\u5316\u5b66\u7684\u5206\u6790\u306f\u3001OBP-301\u304a\u3088\u3073OBP-702\u51e6\u7f6e\u3057\u305f\u816b\u760d\u306e\u4e21\u65b9\u306b\u304a\u3044\u3066MYCN\u767a\u73fe\u306e\u6e1b\u5c11\u30ec\u30d9\u30eb\u3092\u660e\u3089\u304b\u306b\u3057\u305f\uff08\u56f36C\uff09\u3002\u3055\u3089\u306b\u3001\u30b3\u30f3\u30c8\u30ed\u30fc\u30eb\u816b\u760d\u3068\u6bd4\u8f03\u3057\u3066\u3001\u30a6\u30a4\u30eb\u30b9\u51e6\u7406\u3057\u305f\u816b\u760d\u3067\u306f\u3001Ki67\u967d\u6027\u5897\u6b96\u7d30\u80de\u306e\u5272\u5408\u304c\u6709\u610f\u306b\u6e1b\u5c11\u3057\u3066\u3044\u305f\uff08\u56f36D\uff09\u3002\u5bfe\u7167\u7684\u306b\u3001CHP-134\u816b\u760d\u3067\u306f\u3001p53\u767a\u73fe\u306f\u691c\u51fa\u3055\u308c\u306a\u304b\u3063\u305f\uff08\u56f3S8\uff09\u3002\u3053\u308c\u3089\u306e\u7d50\u679c\u306f\u3001OBP-301\u304a\u3088\u3073OBP-702\u306e\u983b\u7e41\u306a\u6295\u4e0e\u304c\u3001MYCN\u5897\u5e45NB\u816b\u760d\u306e\u9664\u53bb\u306b\u304a\u3051\u308b\u4f7f\u7528\u306e\u305f\u3081\u306e\u9ad8\u3044\u6cbb\u7642\u4e0a\u306e\u53ef\u80fd\u6027\u3092\u6709\u3059\u308b\u3053\u3068\u3092\u793a\u5506\u3057\u3066\u3044\u308b\u3002<img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-3206\" src=\"http:\/\/m12n08.jp\/blog\/wp-content\/uploads\/2020\/06\/2020-06-26_165012.jpg\" alt=\"\" width=\"747\" height=\"521\" srcset=\"http:\/\/m12n08.jp\/blog\/wp-content\/uploads\/2020\/06\/2020-06-26_165012.jpg 747w, http:\/\/m12n08.jp\/blog\/wp-content\/uploads\/2020\/06\/2020-06-26_165012-172x120.jpg 172w\" sizes=\"(max-width: 706px) 89vw, (max-width: 767px) 82vw, 740px\" \/><\/p>\n<p>\u56f3 6. OBP-301\u304a\u3088\u3073OBP-702\u306e\u76ae\u4e0bCHP-134\u30bc\u30ce\u30b0\u30e9\u30d5\u30c8\u816b\u760d\u30e2\u30c7\u30eb\u306b\u304a\u3051\u308b\u816b\u760d\u6210\u9577\u6291\u5236\u52b9\u679c<\/p>\n<p><span style=\"font-size: 10pt;\">6\u9031\u9f62\u306e\u96ccBALB\/c nu\/nu\u30de\u30a6\u30b9\u306e\u8107\u8179\u306bCHP-134\u7d30\u80de\uff081\u00d7107\u7d30\u80de\uff09\u3092\u63a5\u7a2e\u3057\u305f\u3002\uff2f\uff22\uff30-\uff13\uff10\uff11\uff08\uff11\uff10\uff18 \uff30\uff26\uff35\uff09\u3001\uff2f\uff22\uff30-\uff17\uff10\uff12\uff08\uff11\uff10\uff18 \uff30\uff26\uff35\uff09\u3001\u307e\u305f\u306f\uff30\uff22\uff33\uff08\u30e2\u30c3\u30af\uff09\u3092\u3001\u9031\u306b\uff13\u56de\u3001\uff13\u30b5\u30a4\u30af\u30eb\u306e\u9593\u3001\u76ae\u5185\u6ce8\u5c04\u3057\u305f\u3002A\uff09\u816b\u760d\u306e\u6210\u9577\u306f\u3001\u5e73\u5747\u816b\u760d\u4f53\u7a4d\u00b1SD\u3068\u3057\u3066\u8868\u3055\u308c\u308b\uff08\u5404\u7fa4\u306en = 10\u307e\u305f\u306f11\uff1b\uff0ap\uff1c0.05\uff09\u3002B\uff09\u5404\u7fa4\u306e\u5168\u5358\u96e2\u816b\u760d\u306e\u5de8\u8996\u7684\u5916\u89b3\u3002C\uff09CHP-134\u816b\u760d\u306e\u7d44\u7e54\u5b66\u7684\u5206\u6790\u3002\u816b\u760d\u306e\u63a5\u7a2e\u304b\u30897\u9031\u9593\u5f8c\u306b\u816b\u760d\u7d44\u7e54\u3092\u5f97\u305f\u3002CHP-134\u816b\u760d\u306e\u30d1\u30e9\u30d5\u30a3\u30f3\u5305\u57cb\u5207\u7247\u3092\u3001\u30d8\u30de\u30c8\u30ad\u30b7\u30ea\u30f3\u304a\u3088\u3073\u30a8\u30aa\u30b7\u30f3\uff08H&amp;E\uff09\u6eb6\u6db2\u3001\u6297MYCN\u6297\u4f53\u3001\u307e\u305f\u306f\u6297Ki67\u6297\u4f53\u3067\u67d3\u8272\u3057\u305f\u3002\u5de6\u3001\u4e2d\u3001\u304a\u3088\u3073\u53f3\u306e\u753b\u50cf\u306f\u3001\u305d\u308c\u305e\u308c\u3001H&amp;E\u3001MYCN\u3001\u304a\u3088\u3073Ki67\u67d3\u8272\u3092\u793a\u3059\u3002\u5404\u56f3\u306e\u5de6\u5074\u306e\u753b\u50cf\u306f\u4f4e\u500d\u7387\u3001\u53f3\u5074\u306e\u753b\u50cf\u306f\u9ed2\u3044\u56db\u89d2\u3067\u7e01\u53d6\u3089\u308c\u305f\u90e8\u5206\u3092\u9ad8\u500d\u7387\u3067\u793a\u3057\u3066\u3044\u307e\u3059\u3002\u5de6\u5074\u306e\u30b9\u30b1\u30fc\u30eb\u30d0\u30fc\u3001200\u03bcm\u3002\u53f3\u5074\u306f100\u03bcm\u3002D\uff09\u816b\u760d\u7d44\u7e54\u4e2d\u306eKi67\u967d\u6027\u7d30\u80de\u306e\u5272\u5408\u3092ImageJ\u30bd\u30d5\u30c8\u30a6\u30a7\u30a2\u3092\u7528\u3044\u3066\u7b97\u51fa\u3057\u305f\u3002\u30c7\u30fc\u30bf\u306f\u5e73\u5747\u5024\u00b1SD\uff08\u5404\u7fa4n=5\uff1b\uff0ap\uff1c0.05\uff09\u3067\u8868\u3057\u3066\u3044\u307e\u3059\u3002<\/span><\/p>\n<hr 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MYCN \u6291\u5236\u306e\u30e1\u30ab\u30cb\u30ba\u30e0\u306b\u3064\u3044\u3066\u306f\u3001OBP-301 \u304a\u3088\u3073 OBP-702 \u611f\u67d3\u3057\u305f NB \u7d30\u80de\u306b\u304a\u3044\u3066\u3001E2F1 \u306e\u30a2\u30c3\u30d7\u30ec\u30ae\u30e5\u30ec\u30fc\u30b7\u30e7\u30f3\u304c MYCN \u767a\u73fe\u3092 mRNA 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p53\u306f\u76f4\u63a5\u7684\u304a\u3088\u3073\u9593\u63a5\u7684\u306a\u30e1\u30ab\u30cb\u30ba\u30e0\u306e\u4e21\u65b9\u3092\u4ecb\u3057\u3066MYCN\u5897\u5e45NB\u816b\u760d\u3067\u306f\u3057\u3070\u3057\u3070\u6a5f\u80fd\u7684\u306b\u4e0d\u6d3b\u6027\u5316\u3055\u308c\u308b\u3002\u76f4\u63a5\u7684\u306a\u4e0d\u6d3b\u6027\u5316\u306e\u30e1\u30ab\u30cb\u30ba\u30e0\u3068\u3057\u3066\u306f\u3001MYCN\u304cp53\u306eC\u672b\u7aef\u9818\u57df\u306b\u7d50\u5408\u3059\u308b\u3053\u3068\u3067\u3001p53\u306e\u4e0b\u6d41\u306b\u3042\u308b\u6a19\u7684\u907a\u4f1d\u5b50\u304c\u4e0d\u6d3b\u6027\u5316\u3055\u308c\u308b31 \u3002\u4e00\u65b9\u3001\u9593\u63a5\u7684\u306a\u30e1\u30ab\u30cb\u30ba\u30e0\u3068\u3057\u3066\u306f\u3001MYCN\u3092\u4ecb\u3057\u305fMDM2\u6d3b\u6027\u5316\u306f\u3001\u30e6\u30d3\u30ad\u30c1\u30f3-\u30d7\u30ed\u30c6\u30a2\u30bd\u30fc\u30e0\u7d4c\u8def\u3092\u4ecb\u3057\u3066MYCN\u5897\u5e45NB\u7d30\u80de\u306b\u304a\u3051\u308bp53\u306e\u767a\u73fe\u3092\u6291\u5236\u3059\u308b32,33\u3002\u3057\u305f\u304c\u3063\u3066\u3001MYCN\u5897\u5e45NB\u7d30\u80de\u306f\u3001E2F1\u3092\u4ecb\u3057\u305fMYCN\u6291\u5236\u306b\u6bd4\u3079\u3066\u3001p53\u3092\u4ecb\u3057\u305fMYCN\u6291\u5236\u306b\u5bfe\u3057\u3066\u611f\u53d7\u6027\u304c\u4f4e\u3044\u53ef\u80fd\u6027\u304c\u3042\u308b\u3002<\/p>\n<p>\u6700\u8fd1\u3001\u5c0f\u5150\u304c\u3093\u3092\u542b\u3080\u69d8\u3005\u306a\u30cf\u30a4\u30ea\u30b9\u30af\u5c0f\u5150\u304c\u3093\u306e\u6cbb\u7642\u306e\u305f\u3081\u306b\u3001\u907a\u4f1d\u5b50\u6539\u5909\u306b\u57fa\u3065\u304f\u500b\u5225\u5316\u533b\u7642\u306e\u30a2\u30d7\u30ed\u30fc\u30c1\u304c\u767b\u5834\u3057\u3066\u3044\u307e\u305934 \u3002\u672c\u7814\u7a76\u3067\u306f\u3001\u30c6\u30ed\u30e1\u30e9\u30fc\u30bc\u3092\u6a19\u7684\u3068\u3057\u305f\u30aa\u30f3\u30b3\u30ed\u30a4\u30c9\u6027\u30a2\u30c7\u30ce\u30a6\u30a4\u30eb\u30b9\u306eMYCN\u6d3b\u6027\u5316NB\u7d30\u80de\u306b\u5bfe\u3059\u308bin vitro\u304a\u3088\u3073in vivo\u3067\u306e\u6cbb\u7642\u52b9\u679c\u3092\u78ba\u8a8d\u3057\u307e\u3057\u305f\u3002MYCN\u306e\u6291\u5236\u306f\u3001\u30a6\u30a4\u30eb\u30b9\u304c\u5a92\u4ecb\u3059\u308b\u6297\u816b\u760d\u52b9\u679c\u306e\u6839\u5e95\u306b\u3042\u308b\u30e1\u30ab\u30cb\u30ba\u30e0\u306b\u304a\u3044\u3066\u91cd\u8981\u306a\u56e0\u5b50\u3067\u3042\u308b\u3002\u6700\u8fd1\u306e\u5305\u62ec\u7684\u306a\u30b2\u30ce\u30e0\u89e3\u6790\u306b\u3088\u308a\u3001MYCN\u5897\u5e4536\u307e\u305f\u306fhTERT\u518d\u914d\u5217\u3092\u793a\u3059\u9ad8\u30ea\u30b9\u30af\u306eNB\u60a3\u8005\u3067\u306fhTERT\u304c\u4e00\u822c\u7684\u306b\u6d3b\u6027\u5316\u3055\u308c\u3066\u3044\u308b\u3053\u3068\u304c\u660e\u3089\u304b\u306b\u306a\u3063\u305f7,8\u3002\u3057\u305f\u304c\u3063\u3066\u3001\u6211\u3005\u304c\u958b\u767a\u3057\u305f\u65b0\u898f\u306ehTERT\u99c6\u52d5\u578b\u30aa\u30f3\u30b3\u30ed\u30a4\u30c9\u30a6\u30a4\u30eb\u30b9\u7642\u6cd5\u306f\u3001MYCN\u5897\u5e45\u307e\u305f\u306fhTERT\u518d\u914d\u5217\u3092\u793a\u3059\u9ad8\u30ea\u30b9\u30af\u306eNB\u7d30\u80de\u3092\u6392\u9664\u3059\u308b\u305f\u3081\u306e\u7279\u306b\u6709\u7528\u306a\u6297\u816b\u760d\u6226\u7565\u3068\u306a\u308a\u3046\u308b\u3002<\/p>\n<p>NB\u816b\u760d\u306f\u3001\u6700\u3082\u983b\u7e41\u306b\u526f\u814e\u304a\u3088\u3073\u4f53\u5185\u306e\u5f8c\u8179\u819c\u75c5\u5909\u304b\u3089\u767a\u751f\u3059\u308b\u3002\u539f\u767a\u6027NB\u816b\u760d\u3092\u8d85\u97f3\u6ce2\u753b\u50cf\u8a3a\u65ad\u307e\u305f\u306f\u30aa\u30f3\u30b3\u30ed\u30a4\u30c9\u6027\u30a2\u30c7\u30ce\u30a6\u30a4\u30eb\u30b9\u306e\u8853\u4e2d\u6ce8\u5c04\u306b\u3088\u308a\u6a19\u7684\u3068\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u308b\u3002\u624b\u8853\u3067\u5207\u9664\u4e0d\u80fd\u306a\u6b8b\u5b58\u816b\u760d\u306b\u3053\u308c\u3089\u306e\u30a6\u30a4\u30eb\u30b9\u3092\u6ce8\u5165\u3059\u308b\u3053\u3068\u306f\u3001\u539f\u767a\u6027\uff2e\uff22\u816b\u760d\u306e\u5c40\u6240\u5236\u5fa1\u306b\u6709\u52b9\u306a\u9078\u629e\u80a2\u304b\u3082\u3057\u308c\u306a\u3044\u3002\u3057\u304b\u3057\u3001MYCN\u3092\u5897\u5e45\u3057\u305fNB\u7d30\u80de\u306f\u3001\u9060\u9694\u81d3\u5668\u306b\u8ee2\u79fb\u6027\u816b\u760d\u3092\u767a\u751f\u3059\u308b\u3053\u3068\u304c\u591a\u304f\u3001\u30aa\u30f3\u30b3\u30ed\u30a4\u30c9\u6027\u30a2\u30c7\u30ce\u30a6\u30a4\u30eb\u30b9\u306e\u816b\u760d\u5185\u6ce8\u5c04\u306f\u4e0d\u53ef\u80fd\u3067\u3042\u308b\u3002\u3057\u305f\u304c\u3063\u3066\u3001\u30a2\u30af\u30bb\u30b9\u3067\u304d\u306a\u3044\u8ee2\u79fb\u6027NB\u816b\u760d\u3092\u6a19\u7684\u3068\u3057\u305f\u6cbb\u7642\u6226\u7565\u3092\u958b\u767a\u3059\u308b\u5fc5\u8981\u304c\u3042\u308b\u3002\u6700\u8fd1\u306e\u8a3c\u62e0\u306f\u3001\u30aa\u30f3\u30b3\u30ed\u30a4\u30c9\u6027\u30a6\u30a4\u30eb\u30b9\u7642\u6cd5\u304c\u514d\u75ab\u539f\u6027\u7d30\u80de\u6b7b\u306e\u8a98\u5c0e\u3092\u4ecb\u3057\u3066\u6297\u816b\u760d\u514d\u75ab\u5fdc\u7b54\u3092\u8a98\u5c0e\u3059\u308b\u3053\u3068\u3092\u793a\u5506\u3057\u3066\u3044\u308b37, 38, 39 \u30aa\u30f3\u30b3\u30ed\u30a4\u30c9\u6027\u30a2\u30c7\u30ce\u30a6\u30a4\u30eb\u30b9\u306f\u3001\u30c0\u30e1\u30fc\u30b8\u306b\u95a2\u9023\u3057\u305f\u5206\u5b50\u30d1\u30bf\u30fc\u30f3\u306e\u653e\u51fa\u3092\u4ecb\u3057\u3066\u514d\u75ab\u539f\u6027\u7d30\u80de\u6b7b\u3092\u8a98\u5c0e\u3059\u308b\u3053\u3068\u304c\u793a\u3055\u308c\u3066\u3044\u308b40\u3002\u6211\u3005\u306f\u6700\u8fd1\u3001hTERT\u99c6\u52d5\u30aa\u30f3\u30b3\u30ea\u30fc\u6027\u30a2\u30c7\u30ce\u30a6\u30a4\u30eb\u30b9\u3092\u539f\u767a\u90e8\u4f4d\u306b\u76ae\u5185\u6ce8\u5c04\u3059\u308b\u3053\u3068\u3067\u3001\u809d\u81d3\u306e\u8ee2\u79fb\u6027\u5927\u8178\u76f4\u8178\u816b\u760d\u306b\u304a\u3051\u308b\u514d\u75ab\u30c1\u30a7\u30c3\u30af\u30dd\u30a4\u30f3\u30c8\u963b\u5bb3\u5264\u306e\u6cbb\u7642\u52b9\u679c\u304c\u9ad8\u307e\u308b\u3053\u3068\u3092\u660e\u3089\u304b\u306b\u3057\u305f41\u3002<\/p>\n<p>\u7d50\u8ad6\u3068\u3057\u3066\u3001\u30c6\u30ed\u30e1\u30e9\u30fc\u30bc\u3092\u6a19\u7684\u3068\u3057\u305f\u30aa\u30f3\u30b3\u30ed\u30a4\u30c9\u6027\u30a2\u30c7\u30ce\u30a6\u30a4\u30eb\u30b9OBP-301\u304a\u3088\u3073OBP-702\u306e\u4f7f\u7528\u306f\u3001MYCN\u306e\u6291\u5236\u3092\u4ecb\u3057\u305fMYCN\u5897\u5e45\u6027NB\u816b\u760d\u306e\u6cbb\u7642\u306b\u6709\u671b\u306a\u6cbb\u7642\u6cd5\u3067\u3042\u308b\u3053\u3068\u3092\u793a\u3057\u305f\u3002\u30c6\u30ed\u30e1\u30e9\u30fc\u30bc\u3092\u6a19\u7684\u3068\u3057\u305f\u30aa\u30f3\u30b3\u30ed\u30a4\u30c9\u30a6\u30a4\u30eb\u30b9\u7642\u6cd5\u306e\u5b89\u5168\u6027\u3068\u5b9f\u884c\u53ef\u80fd\u6027\u3092\u8abf\u3079\u308b\u305f\u3081\u306b\u3001MYCN\u3068hTERT\u306e\u904e\u5270\u767a\u73fe\u3092\u793a\u3059\u9ad8\u30ea\u30b9\u30af\u306eNB\u60a3\u8005\u3092\u5bfe\u8c61\u3068\u3057\u305f\u66f4\u306a\u308b\u81e8\u5e8a\u7814\u7a76\u304c\u5fc5\u8981\u3067\u3042\u308b\u3002<\/p>\n<h2>\u6750\u6599\u3068\u65b9\u6cd5<\/h2>\n<p>\u30bb\u30eb\u30e9\u30a4\u30f3<\/p>\n<p>\uff08\u3068\u308a\u3042\u3048\u305a\u3053\u3053\u307e\u3067\uff09by \u30b7\u30a8\u30f3<\/p>\n<div class=\"pt-cv-wrapper\"><div class=\"pt-cv-view pt-cv-grid pt-cv-colsys pt-cv-post-border pt-cv-left\" id=\"pt-cv-view-15185e6rup\"><div data-id=\"pt-cv-page-1\" class=\"pt-cv-page\" data-cvc=\"1\"><div class=\"col-md-12 col-sm-12 col-xs-12 pt-cv-content-item pt-cv-2-col\"  data-pid=\"2483\"><div class='pt-cv-ifield'><h4 class=\"pt-cv-title\"><a href=\"http:\/\/m12n08.jp\/blog\/share\/4588oncolys\/2483.html\" class=\"_self cvplbd\" target=\"_self\" >4588\u30aa\u30f3\u30b3\u30ea\u30b9\u30d0\u30a4\u30aa\u30d5\u30a1\u30fc\u30de\u306e\u5c06\u6765\u6027\uff0f\u304c\u3093\u3092\u5207\u3089\u305a\u306b\u6cbb\u3059 \u30a6\u30a4\u30eb\u30b9\u7642\u6cd5\u30c6\u30ed\u30e1\u30e9\u30a4\u30b7\u30f3<\/a><\/h4>\n<a href=\"http:\/\/m12n08.jp\/blog\/share\/4588oncolys\/2483.html\" class=\"_self pt-cv-href-thumbnail pt-cv-thumb-left cvplbd\" target=\"_self\" ><img loading=\"lazy\" decoding=\"async\" width=\"200\" height=\"120\" src=\"http:\/\/m12n08.jp\/blog\/wp-content\/uploads\/2019\/04\/2019-05-01_152331-200x120.png\" class=\"pt-cv-thumbnail img-shadow pull-left\" alt=\"\u30aa\u30f3\u30b3\u30ea\u30b9 \u30d3\u30b8\u30cd\u30b9\u30e2\u30c7\u30eb\" srcset=\"http:\/\/m12n08.jp\/blog\/wp-content\/uploads\/2019\/04\/2019-05-01_152331-200x120.png\" sizes=\"100vw\" wpfc-lazyload-disable=\"true\" \/><\/a>\n<div class=\"pt-cv-content\">\u3053\u3093\u306b\u3061\u306f\u3001\u30b7\u30a8\u30f3\uff08@m12n08.jp\uff09\u3067\u3059\u3002 \u3010\u672c\u30da\u30fc\u30b8\u306f2019\u5e744\u6708\u306b\u66f8\u3044\u305f\u8a18\u4e8b\u30922021\u5e741\u6708\u306b\u52a0\u7b46\u3057\u307e\u3057\u305f\u3011 2019\u5e7412\u670830\u65e5\uff08\u6708\uff09\u6642\u70b9\u30674588\u30aa\u30f3\u30b3\u30ea\u30b9\u309215,500\u682a\u4fdd\u6709\u3057\u3066\u3044\u307e\u3059\uff08\u5e73\u5747\u53d6\u5f97\u4fa1\u683c\u7d042,035\u5186\uff09\u3002 \u3082\u3057\u30aa\u30f3\u30b3\u30ea\u30b9\u306e\u682a\u4fa1\u304c30,000\u5186\u306b\u306a\u308c\u3070\u79c1\u306e\u682a\u8cc7\u7523\u306f4.65\u5104\u5186\u3068\u306a\u308a\u307e\u3059\u3002 \u306a\u308b\u3053\u3068\u306f\u9593\u9055\u3044\u306a\u3044\u3068\u500b\u4eba\u7684\u306b\u601d\u3063\u3066\u304a\u308a\u3001\u554f\u984c\u306f\u300c\u3044\u3064\u304b\u300d\u3068\u3044\u3046\u3053\u3068\u3067\u3059\u30fb\u30fb\u30fb\u3002 \u3055<br \/><div class=\"pt-cv-rmwrap\"><a href=\"http:\/\/m12n08.jp\/blog\/share\/4588oncolys\/2483.html\" class=\"_self pt-cv-readmore btn btn-success cvplbd\" target=\"_self\">\uff1e\uff1e\u7d9a\u304d\u3092\u8aad\u3080<\/a><\/div><\/div>\n<div class=\"pt-cv-meta-fields\"><span class=\"terms\"><span class=\"glyphicon glyphicon-folder-open\"><\/span> <a href='http:\/\/m12n08.jp\/blog\/.\/share\/4588oncolys' title='4588\u30aa\u30f3\u30b3\u30ea\u30b9' class='pt-cv-tax-4588oncolys'>4588\u30aa\u30f3\u30b3\u30ea\u30b9<\/a><\/span><\/div><\/div><\/div><\/div><\/div><\/div>\t\t\t<style type=\"text\/css\" id=\"pt-cv-inline-style-dbcdcfemei\">#pt-cv-view-15185e6rup.pt-cv-post-border { margin: 0; border-top-width: 2px; border-left-width: 2px }\n#pt-cv-view-15185e6rup.pt-cv-post-border { margin: 0; border-top-style: groove; border-left-style: groove }\n#pt-cv-view-15185e6rup.pt-cv-post-border { margin: 0; border-top-color: #1122cc; border-left-color: #1122cc }\n#pt-cv-view-15185e6rup.pt-cv-post-border .pt-cv-content-item   { border-right-width: 2px; border-bottom-width: 2px; border-right-style: groove; border-bottom-style: groove; border-right-color: #1122cc; border-bottom-color: #1122cc; }\n#pt-cv-view-15185e6rup .pt-cv-title a, #pt-cv-view-15185e6rup  .panel-title { font-size: 15px !important; line-height: 1.3 !important; color: #1122cc !important; font-weight: 600 !important; }\n#pt-cv-view-15185e6rup  .pt-cv-title a:hover, #pt-cv-view-15185e6rup  .panel-title:hover   { color: #ff89ff !important; }\n#pt-cv-view-15185e6rup .pt-cv-meta-fields * { font-weight: 600 !important; }\n#pt-cv-view-15185e6rup .pt-cv-readmore  { color: #ffffff !important; background-color: #1122cc !important; }\n#pt-cv-view-15185e6rup .pt-cv-tao  { font-size: 20px !important; line-height: 1.3 !important; }\n#pt-cv-view-15185e6rup { text-align: left; }<\/style>\r\n\t\t\t\n<div class=\"pt-cv-wrapper\"><div class=\"pt-cv-view pt-cv-grid pt-cv-colsys pt-cv-post-border pt-cv-left\" id=\"pt-cv-view-46cddees9p\"><div data-id=\"pt-cv-page-1\" class=\"pt-cv-page\" data-cvc=\"1\"><div class=\"col-md-12 col-sm-12 col-xs-12 pt-cv-content-item pt-cv-2-col\"  data-pid=\"3061\"><div class='pt-cv-ifield'><h4 class=\"pt-cv-title\"><a href=\"http:\/\/m12n08.jp\/blog\/share\/4588oncolys\/3061.html\" class=\"_self cvplbd\" target=\"_self\" >4588\u30aa\u30f3\u30b3\u30ea\u30b9\u3092\u9577\u671f\u4fdd\u6709\u3059\u308b\u305f\u3081\u306e\u77e5\u8b58\u30fb\u8cc7\u6599<\/a><\/h4>\n<a href=\"http:\/\/m12n08.jp\/blog\/share\/4588oncolys\/3061.html\" class=\"_self pt-cv-href-thumbnail pt-cv-thumb-left cvplbd\" target=\"_self\" ><img loading=\"lazy\" decoding=\"async\" width=\"200\" height=\"120\" src=\"http:\/\/m12n08.jp\/blog\/wp-content\/uploads\/2020\/06\/2021-01-24_164006-200x120.png\" class=\"pt-cv-thumbnail img-shadow pull-left\" alt=\"\u30aa\u30f3\u30b3\u30ea\u30b9\u30d0\u30a4\u30aa\u30d5\u30a1\u30fc\u30de \u30c6\u30ed\u30e1\u30e9\u30a4\u30b7\u30f3 \u30d1\u30a4\u30d7\u30e9\u30a4\u30f3\u4e00\u89a7 \u5c06\u6765\u6027\" srcset=\"http:\/\/m12n08.jp\/blog\/wp-content\/uploads\/2020\/06\/2021-01-24_164006-200x120.png\" sizes=\"100vw\" wpfc-lazyload-disable=\"true\" \/><\/a>\n<div class=\"pt-cv-content\">\u3053\u3093\u306b\u3061\u306f\u3001\u30b7\u30a8\u30f3\uff08@M12n08Jp)\u3067\u3059\u3002 \u3053\u306e\u30da\u30fc\u30b8\u3067\u306f4588\u30aa\u30f3\u30b3\u30ea\u30b9\u30d0\u30a4\u30aa\u30d5\u30a1\u30fc\u30de\u95a2\u9023\u3067\u545f\u3044\u305f\u8cc7\u6599\u3092\u4e2d\u5fc3\u306b\u30aa\u30f3\u30b3\u30ea\u30b9\u793e\u306e<br \/><div class=\"pt-cv-rmwrap\"><a href=\"http:\/\/m12n08.jp\/blog\/share\/4588oncolys\/3061.html\" class=\"_self pt-cv-readmore btn btn-success cvplbd\" target=\"_self\">\uff1e\uff1e\u7d9a\u304d\u3092\u8aad\u3080<\/a><\/div><\/div>\n<div class=\"pt-cv-meta-fields\"><span class=\"terms\"><span class=\"glyphicon glyphicon-folder-open\"><\/span> <a href='http:\/\/m12n08.jp\/blog\/.\/share\/4588oncolys' title='4588\u30aa\u30f3\u30b3\u30ea\u30b9' class='pt-cv-tax-4588oncolys'>4588\u30aa\u30f3\u30b3\u30ea\u30b9<\/a><\/span><\/div><\/div><\/div><\/div><\/div><\/div>\t\t\t<style type=\"text\/css\" id=\"pt-cv-inline-style-116195cdqv\">#pt-cv-view-46cddees9p.pt-cv-post-border { margin: 0; border-top-width: 2px; border-left-width: 2px }\n#pt-cv-view-46cddees9p.pt-cv-post-border { margin: 0; border-top-style: groove; border-left-style: groove }\n#pt-cv-view-46cddees9p.pt-cv-post-border { margin: 0; border-top-color: #1122cc; border-left-color: #1122cc }\n#pt-cv-view-46cddees9p.pt-cv-post-border .pt-cv-content-item   { border-right-width: 2px; border-bottom-width: 2px; border-right-style: groove; border-bottom-style: groove; border-right-color: #1122cc; border-bottom-color: #1122cc; }\n#pt-cv-view-46cddees9p .pt-cv-title a, #pt-cv-view-46cddees9p  .panel-title { font-size: 15px !important; line-height: 1.3 !important; color: #1122cc !important; font-weight: 600 !important; }\n#pt-cv-view-46cddees9p  .pt-cv-title a:hover, #pt-cv-view-46cddees9p  .panel-title:hover   { color: #ff89ff !important; }\n#pt-cv-view-46cddees9p .pt-cv-meta-fields * { font-weight: 600 !important; }\n#pt-cv-view-46cddees9p .pt-cv-readmore  { color: #ffffff !important; background-color: #1122cc !important; }\n#pt-cv-view-46cddees9p .pt-cv-tao  { font-size: 20px !important; line-height: 1.3 !important; }\n#pt-cv-view-46cddees9p { text-align: left; }<\/style>\r\n\t\t\t\n<div class=\"pt-cv-wrapper\"><div class=\"pt-cv-view pt-cv-grid pt-cv-colsys pt-cv-post-border pt-cv-left\" id=\"pt-cv-view-5db66d9qdh\"><div data-id=\"pt-cv-page-1\" class=\"pt-cv-page\" data-cvc=\"1\"><div class=\"col-md-12 col-sm-12 col-xs-12 pt-cv-content-item pt-cv-2-col\"  data-pid=\"2887\"><div class='pt-cv-ifield'><h4 class=\"pt-cv-title\"><a href=\"http:\/\/m12n08.jp\/blog\/share\/4588oncolys\/2887.html\" class=\"_self cvplbd\" target=\"_self\" >4588\u30aa\u30f3\u30b3\u30ea\u30b9\u30d0\u30a4\u30aa\u30d5\u30a1\u30fc\u30de\uff0f2013\u5e74\u4e0a\u5834\u6642\u306e\u6d66\u7530\u793e\u9577\u30a4\u30f3\u30bf\u30d3\u30e5\u30fc<\/a><\/h4>\n<a href=\"http:\/\/m12n08.jp\/blog\/share\/4588oncolys\/2887.html\" class=\"_self pt-cv-href-thumbnail pt-cv-thumb-left cvplbd\" target=\"_self\" ><img loading=\"lazy\" decoding=\"async\" width=\"200\" height=\"120\" src=\"http:\/\/m12n08.jp\/blog\/wp-content\/uploads\/2019\/12\/2019-12-22_171820-200x120.png\" class=\"pt-cv-thumbnail img-shadow pull-left\" alt=\"\u30aa\u30f3\u30b3\u30ea\u30b9 \u30c6\u30ed\u30e1\u30e9\u30a4\u30b7\u30f3 \u6d66\u7530\u793e\u9577\" srcset=\"http:\/\/m12n08.jp\/blog\/wp-content\/uploads\/2019\/12\/2019-12-22_171820-200x120.png\" sizes=\"100vw\" wpfc-lazyload-disable=\"true\" \/><\/a>\n<div class=\"pt-cv-content\">\u3053\u3093\u306b\u3061\u306f\u3001\u30b7\u30a8\u30f3\uff08@M12n08Jp)\u3067\u3059\u3002 \u3053\u306e\u30da\u30fc\u30b8\u3067\u306f\u30aa\u30f3\u30b3\u30ea\u30b9\u30d0\u30a4\u30aa\u30d5\u30a1\u30fc\u30de \u6d66\u7530\u793e\u9577\u304c\u4e0a\u5834\u6642\u306b\u65e5\u672c\u30d9\u30f3\u30c1\u30e3\u30fc\u30ad\u30e3\u30d4\u30bf\u30eb\u306e\u53d6\u6750\u306b\u5fdc\u3058\u3066\u53d7\u3051\u3089\u308c\u305f\u30a4\u30f3\u30bf\u30d3\u30e5\u30fc\u3092\u7d39\u4ecb\u81f4\u3057\u307e\u3059\uff08\u30cd\u30c3\u30c8\u3067\u62fe\u3063\u3066\u304d\u307e\u3057\u305f\uff09\u3002 \u30aa\u30f3\u30b3\u30ea\u30b9\u30d0\u30a4\u30aa\u30d5\u30a1\u30fc\u30de\u306e\u5275\u696d\u671f\u306e\u82e6\u52b4\u3092\u77e5\u308b\u3053\u3068\u304c\u51fa\u6765\u307e\u3059\u3002 <br \/><div class=\"pt-cv-rmwrap\"><a href=\"http:\/\/m12n08.jp\/blog\/share\/4588oncolys\/2887.html\" class=\"_self pt-cv-readmore btn btn-success cvplbd\" target=\"_self\">\uff1e\uff1e\u7d9a\u304d\u3092\u8aad\u3080<\/a><\/div><\/div>\n<div class=\"pt-cv-meta-fields\"><span class=\"terms\"><span class=\"glyphicon glyphicon-folder-open\"><\/span> <a href='http:\/\/m12n08.jp\/blog\/.\/share\/4588oncolys' title='4588\u30aa\u30f3\u30b3\u30ea\u30b9' class='pt-cv-tax-4588oncolys'>4588\u30aa\u30f3\u30b3\u30ea\u30b9<\/a><\/span><\/div><\/div><\/div><\/div><\/div><\/div>\t\t\t<style type=\"text\/css\" id=\"pt-cv-inline-style-5d9b40372g\">#pt-cv-view-5db66d9qdh.pt-cv-post-border { margin: 0; border-top-width: 2px; border-left-width: 2px }\n#pt-cv-view-5db66d9qdh.pt-cv-post-border { margin: 0; border-top-style: groove; border-left-style: groove }\n#pt-cv-view-5db66d9qdh.pt-cv-post-border { margin: 0; border-top-color: #1122cc; border-left-color: #1122cc }\n#pt-cv-view-5db66d9qdh.pt-cv-post-border .pt-cv-content-item   { border-right-width: 2px; border-bottom-width: 2px; border-right-style: groove; border-bottom-style: groove; border-right-color: #1122cc; border-bottom-color: #1122cc; }\n#pt-cv-view-5db66d9qdh .pt-cv-title a, #pt-cv-view-5db66d9qdh  .panel-title { font-size: 15px !important; line-height: 1.3 !important; color: #1122cc !important; font-weight: 600 !important; }\n#pt-cv-view-5db66d9qdh  .pt-cv-title a:hover, #pt-cv-view-5db66d9qdh  .panel-title:hover   { color: #ff89ff !important; }\n#pt-cv-view-5db66d9qdh .pt-cv-meta-fields * { font-weight: 600 !important; }\n#pt-cv-view-5db66d9qdh .pt-cv-readmore  { color: #ffffff !important; background-color: #1122cc !important; }\n#pt-cv-view-5db66d9qdh .pt-cv-tao  { font-size: 20px !important; line-height: 1.3 !important; }\n#pt-cv-view-5db66d9qdh { text-align: left; }<\/style>\r\n\t\t\t\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p><script async src=\"https:\/\/platform.twitter.com\/widgets.js\" charset=\"utf-8\"><\/script><\/p>\n<div class=\"ninja_onebutton\"><script type=\"text\/javascript\">\n\/\/<![CDATA[\n(function(d){\nif(typeof(window.NINJA_CO_JP_ONETAG_BUTTON_9554728d8361e45dac59302c616ca4e1)=='undefined'){\n    document.write(\"<sc\"+\"ript type='text\/javascript' src='\/\/omt.shinobi.jp\/b\/9554728d8361e45dac59302c616ca4e1'><\/sc\"+\"ript>\");\n}else{\n    window.NINJA_CO_JP_ONETAG_BUTTON_9554728d8361e45dac59302c616ca4e1.ONETAGButton_Load();}\n})(document);\n\/\/]]>\n<\/script><!--?php the_permalink(); ?--><!--?php the_title(); ?--><\/div>\n","protected":false},"excerpt":{"rendered":"<p>\u3053\u3093\u306b\u3061\u306f\u3001\u30b7\u30a8\u30f3\uff08@M12n08Jp)\u3067\u3059\u3002 IR\u3055\u308c\u305f\u8ad6\u6587\u306eDeepL\u3067\u306e\u7ffb\u8a33\u3067\u3059\u3002 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